Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157 : H7 derived from the Sakai outbreak
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概要
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The enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain RIMD 0509952, derived from an outbreak in Sakai city, Japan, in 1996, produces two kinds of verotoxins, VT1 and VT2, encoded by the stx1 and stx2 genes. In the EHEC strains, as well as in other VT-producing E. coli strains, the toxins are encoded by lysogenic bacteriophages. The EHEC O157:H7 strain RIMD 0509952 did not produce plaque-forming phage particles upon inducing treatments. We have determined the complete nucleotide sequence of a prophage, VT2-Sakai, carrying the stx2A and stx2B genes on the chromosome, and presumed the putative functions of the encoded proteins and the cis-acting DNA elements based on sequence homology data. To our surprise, the sequences in the regions of VT2-Sakai corresponding to the early gene regulators and replication proteins, and the DNA sequences recognized by the regulators share very limited homology to those of the VT2-encoding 933W phage carried by the EHEC O157:H7 strain EDL933 reported by Plunkett et al. (J. Bacteriol., p1767-1778, 181, 1999), although the sequences corresponding to the structural components are almost identical. These data suggest that these two phages were derived from a common ancestral phage and that either or both of them underwent multiple genetic rearrangements. An IS629 insertion was found downstream of the stx2B gene and upstream of the lysis gene S, and this might be responsible for the absence of plaque-forming activity in the lysate obtained after inducing treatments.
- 日本遺伝学会の論文
著者
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HAYASHI Tetsuya
Department of Internal Medicine III, Osaka Medical College
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Honda Takeshi
Department of Bacterial Infections, Research Institute for Microbial Disease, Osaka University
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ABE Hiroyuki
Department of Applied Physics, Graduate School of Engineering, Tohoku University
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Kurokawa Ken
Genome Information Research Center Osaka University:research Institute Of Microbial Diseases Osaka U
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Kurokawa Ken
Genome Information Research Center Osaka University
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Abe Hiroyuki
Department Of Bacteriology Institute Of Medical Science The University Of Tokyo
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Abe Hiroyuki
Department Of Applied Chemistry Faculty Of Engineering The University Of Tokyo
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TATSUNO Ichiro
Department of Clinical Cell Biology (Second Department of Medicine), Chiba University Graduate Schoo
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ONISHI Makoto
Department of New Materials Section, Terumo Research and Development Center,
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Onishi Makoto
Department Of Bacteriology School Of Medicine Shinsu University
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SASAKAWA Chihiro
Department of Bacterial Infection, Institute of Medical Science, The University of Tokyo
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Iida Tetsuya
Department Of Bacterial Infections Research Institute For Microbial Diseases Osaka University
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Shinagawa Hideo
Department of Biomedical Sciences, University of Maryland Baltimore
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Honda Takeshi
Department Of Bacterial Infections Research Institute For Microbial Disease Osaka University
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YASUNAGA Teruo
Genome Information Research Center, Osaka University
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Yasunaga Teruo
Genome Information Research Center Osaka University
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Tatsuno Ichiro
Department Of Bacteriology Institute Of Medical Science The University Of Tokyo
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MAKINO Kozo
Department of Experimental Chemotherapy, The Research Institute for Microbial Disease, Osaka Univers
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Makino Kozo
Department Of Applied Chemistry National Defense Academy
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Makino Kozo
Department Of Molecular Microbiology Research Institute For Microbial Diseases Osaka University
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Hayashi Tetsuya
Department Of Bacteriology School Of Medicine Shinsu University
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YOKOYAMA Katsushi
Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University
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KUBOTA Yoshino
Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University
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YUTSUDO Chikako
Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University
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KIMURA Sigenobu
Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University
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ISHII Kazuo
Human Genome Research Group, RIKEN Genomic Science Center, c/o Kitasato University
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HATTORI Masahiro
Human Genome Research Group, RIKEN Genomic Science Center, c/o Kitasato University
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YAMAMOTO Koichiro
Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University
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Kubota Yoshino
Department Of Molecular Microbiology Research Institute For Microbial Diseases Osaka University
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Kimura Sigenobu
Department Of Molecular Microbiology Research Institute For Microbial Diseases Osaka University
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Yutsudo Chikako
Department Of Molecular Microbiology Research Institute For Microbial Diseases Osaka University
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Shinagawa Hideo
Department Of Biomedical Sciences University Of Maryland Baltimore
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Yamamoto Koichiro
Department Of Bacterial Infections Research Institute For Microbial Diseases Osaka University
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Yokoyama Katsushi
Department Of Molecular Microbiology Research Institute For Microbial Diseases Osaka University
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Sasakawa Chihiro
Department Of Bacteriology Institute Of Medical Science The University Of Tokyo
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Sasakawa Chihiro
Department Of Bacterial Infection Institute Of Medical Science The University Of Tokyo
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Iida Tetsuya
Department Of Bacterial Infections Research Institute For Microbial Disease Osaka University
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Honda Takeshi
Department Of Bacterial Infections Research Institute For Microbial Diseases Osaka University
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Ishii Kazuo
Human Genome Research Group Riken Genomic Science Center C/o Kitasato University
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Hattori Masahiro
Human Genome Research Group Riken Genomic Science Center C/o Kitasato University
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