In Planta Production of Immunogenic Poliovirus Peptide Using Tobacco Mosaic Virus-Based Vector System(PLANT BIOTECHNOLOGY)

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概要

• 論文の詳細を見る

• The tobacco mosaic virus (TMV) provides an attractive means of producing foreign peptides in plants. In this study, a TMV-based vector was designed such that a fragment encoding 15 amino acids of the poliovirus peptide (PVP) derived from the viral capsid proteins VP3 and VP1 of poliovirus type 1 Sabin was inserted downstream of the six-base 3' context nucleotide sequence of the TMV coat protein (CP) gene. This design allowed readthrough at the amber stop codon, thereby producing the chimeric TMV particle with both intact CP and CP-fusion protein (CP-PVP) in Nicotiana tabacum cv. Samsun infected with the TMV vector. The TMVCP-PVP virus particle induced antibodies against PVP as well as TMVCP in mice after intraperitoneal immunization. These data illustrate the potential of the readthrough translation system with TMVCP for antigen presentation and vaccine production.

• 社団法人日本生物工学会の論文
• 2006-05-25

著者

• Fujiyama Kazuhito

  International Center for Biotechnology, Osaka University

• Fujiyama Kazuhito

  The International Center for Biotechnology, Osaka University

• MISAKI Ryo

  The International Center for Biotechnology, Osaka University

• SEKI Tatsuji

  The International Center for Biotechnology, Osaka University

• YANAGIHARA Itaru

  Department of Environmental Medicine, Osaka Medical Center and Research Institute for Maternal and C

• Yanagihara Itaru

  Department Of Bacterial Infections Research Institute For Microbial Diseases Osaka University

• Saejung Wanida

  The International Center for Biotechnology, Osaka University

• Nakado Junko

  The International Center for Biotechnology, Osaka University

• Honda Takeshi

  Department of Bacterial Infections, Research Institute for Microbial Disease, Osaka University

• Watanabe Yuichiro

  Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo

• SEKI Tatsuji

  International Center for Biotechnology, Osaka University

• Kazuhito Fujiyama

  International Center For Biotechnology Osaka University

• Fujiyama Kazuhito

  大阪大学生物工学国際交流センター

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka Univ.

• Fujiyama Kazuhito

  Department Of Fermentation Technology Osaka University

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka University

• Misaki Ryo

  International Center For Biotechnology Osaka University

• Misaki Ryo

  The International Center For Biotechnology Osaka University

• Seki Tatsuji

  International Center For Biotechnology Osaka University

• Seki Tatsuji

  The International Center For Biotechnology Osaka University

• Seki Tatsuji

  大阪大学生物工学国際交流センター

• Honda T

  Department Of Bacterial Infections Research Institute For Microbial Disease Osaka University

• Honda T

  Department Of Bacterial Infection Research Institute For Microbial Diseases Osaka University

• Honda Takeshi

  Department Of Bacterial Infections Research Institute For Microbial Disease Osaka University

• Nakado Junko

  The International Center For Biotechnology Osaka University

• Saejung Wanida

  The International Center For Biotechnology Osaka University

• Fujiyama Kazuhito

  The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn

• Yanagihara Itaru

  Department Of Developmental Infectious Diseases Research Institute Osaka Medical Center For Maternal

• Honda Takeshi

  Research Institute For Microbial Diseases Osaka University

• Honda Takeshi

  Department Of Bacterial Infections Research Institute For Microbial Diseases Osaka University

• Watanabe Yuichiro

  Department Of Life Science Graduate School Of Arts And Science The University Of Tokyo

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