Molecular Cloning and Gene Expression Analysis of Tomato Endo-β-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening
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概要
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In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the high-mannose type N-glycans with the Manα1-2Manα1-3Manβ1-4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.
- 社団法人 日本農芸化学会の論文
著者
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Fujiyama Kazuhito
The International Center for Biotechnology, Osaka University
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INOUE Masami
Department of Hematology/Oncology, Osaka Medical Center, Research Institute for Maternal and Child H
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KIMURA Yoshinobu
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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MAEDA Megumi
Department of Hygiene, Kawasaki Medical School
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Nakamura Kosuke
Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama Un
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NAKANO Ryohei
Department of Plant Functions and Regulation, Graduate School of Natural Science and Technology, Oka
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HOSOI Katsutoshi
Research Institute, Kagome Co., Ltd.
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Maeda Megumi
Okayama Univ. Okayama
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