Structural Features of N-Glycans Linked to Royal Jelly Glycoproteins : Structures of high-mannose type, hybrid type, and biantennary type glycans

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概要

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• The structures of N-glycans of total glycoproteins in royal jelly have been explored to clarify whether antigenic N-glycans occur in the famous health food. The structural feature of N-glycans linked to glycoproteins in royal jelly was first characterized by immunoblotting with an antiserum against plant complex type N-glycan and lectin-blotting with Con A and WGA. For the detail structural analysis of such N-glycans, the pyridylaminated (PA-) N-glycans were prepared from hydrazinolysates of total glycoproteins in royal jelly and each PA-sugar chain was purified by reverse-phase HPLC and size-fractionation HPLC. Each structure of the PA-sugar chains purified was identified by the combination of two-dimensional PA-sugar chain mapping, ESI-MS and MS/MS analyses, sequential exoglycosidase digestions, and 500 MHz ^1H-NMR spectrometry. The immunoblotting and lectinblotting analyses preliminarily suggested the absence of antigenic N-glycan bearing β1-2 xylosyl and/or α1-3 fucosyl residue (s) and occurrence of β1-4GlcNAc residue in the insect glycoproteins. The detailed structural analysis of N-glycans of total royal jelly glycoproteins revealed that the antigenic N-glycans do not occur but the typical high mannose-type structure (Man_<9-4>GlcNAc_2) occupies 71.6% of total N-glycan, biantennary-type structures (GlcNAc_2Man_3GlcNAc_2) 8.4%, and hybrid type structure (GlcNAc_1Man_4GlcNAc_2) 3.0%. Although the complete structures of the remaining 17% N-glycans ; C4,(HexNAc_3Hex_3HexNAc_2 : 3.0%), D2 (HexNAc_2Hex_5HexNAc_2 : 4.5%), and D3 (HexNAc_3Hex_4HexNAc_2 : 9.5%) are still obscure so far, ESI-MS analysis, exoglycosidase digestions by two kinds of β-N-acetylglucosaminidase, and WGA blotting suggested that these N-glycans might bear a β1-4 linkage N-acetylglucosaminyl residue.

• 社団法人日本農芸化学会の論文
• 2000-10-23

著者

• 木村 吉伸

  岡山大学農学部

• 木村 吉伸

  岡山大学 農

• Sugimoto Hiroyuki

  Yamada Apiculture Center, Inc.

• KIMURA Yoshinobu

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• 木村 吉伸

  Dep. Of Biofunctional Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ.

• Kuroyanagi M

  Fac. Of Pharmaceutical Sciences At Kagawa Campus Tokushima Bunri Univ.

• 木村 吉伸

  岡山大学

• Nitoda Teruhiko

  Department of Environmental Science, Policy and Management, University of California

• Kimura Mariko

  Department Of Breast Oncology Aichi Cancer Center Hospital

• Kimura Mariko

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Nitoda Teruhiko

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Kimura Yoshinobu

  Department Of Agricultural Science Faculty Of Agriculture Okayama University

• Sugimoto Hiroyuki

  Yamada Apiculture Center

• MIYAGI Chiyoko

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• KAWAI Nobuyuki

  Yamada Bee Farm

• Kuroyanagi Masanori

  Fac. Of Pharmaceutical Sciences At Kagawa Campus Tokushima Bunri Univ.

• Miyagi Chiyoko

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Kimura Mariko

  Fac. Of Food Culture Dep. Of Nutrition Kurashiki Sakuyo Univ.

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