First Evidence for Occurrence of Galβ1-3GlcNAcβ1-4Man Unit in N-Glycans of Insect Glycoprotein : β1-3Gal and β1-4GlcNAc Transferases Are Involved in N-Glycan Processing of Royal Jelly Glycoproteins(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
On a way of structural analysis of total N-glycans linked to glycoproteins in royal jelly (Kimura, Y. et al., Biosci. Biotechnol. Biochem., 64, 2109-2120 (2000), Kimura, M. et al., Biosci. Biotechnol. Biochem., 66, 1985-1989 (2002)), we found that some complex type N-glycans containing a β1-3galactose residue occur on the insect glycoproteins. Up to date, it has been considered that naturally occurring insect glycoproteins do not bear the galactose-containing N-glycans, therefore, in this report we describe the structural analysis of the complex type N-glycans of royal jelly glycoproteins. By a combination of endo- and exo-glycosidase digestions, IS-MS analysis, and ^1H-NMR spectroscopy, the structures of the β1-3 galactose-containing N-glycan were identified as the following ; GlcNAcβ1-2Manα1-6 [GlcNAcβ1-2 (Galβ1-3GlcNAcβ1-4) Manα1-3] Manβ1-4GlcNAcβ1-4GlcNAc, Manα1-3Manα1-6 [GlcNAcβ1-2 (Galβ1-3GlcNAcβ1-4) Manα1-3] Manβ1-4GlcNAcβ1-4GlcNAc, and Manα1-6 (Manα1-3) Manα1-6 [GlcNAcβ1-2 (Galβ1-3GlcNAcβ1-4) Manα1-3] Manβ1-4GlcNAcβ1-4GlcNAc. To our knowledge, this is the first report showing that the Galβ1-3GlcNAcβ1-4Man unit occurs in N-glycans of insect glycoproteins, indicating a β1-3 galactosyl transferase and β1-4GlcNAc transferase (GNT-IV) are expressed in the honeybee cells.
- 社団法人日本農芸化学会の論文
- 2003-08-23
著者
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木村 吉伸
岡山大学農学部
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木村 吉伸
岡山大学 農
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Sugimoto Hiroyuki
Yamada Apiculture Center, Inc.
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YAMADA Hideo
Yamada Apiculture Center, Inc.
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KIMURA Yoshinobu
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Yamada Hideyuki
SEIREN CO. LTD.
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木村 吉伸
Dep. Of Biofunctional Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ.
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Kimura Mariko
Faculty of Food Culture, Department of Nutrition, Kurashiki Sakuyo University
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Kuroyanagi M
Fac. Of Pharmaceutical Sciences At Kagawa Campus Tokushima Bunri Univ.
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木村 吉伸
岡山大学
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Kimura Mariko
Faculty Of Food Culture Department Of Food Systems Kurashiki Sakuyo University
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Kimura M
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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OKIHARA Kiyoshi
Yamada Apiculture Center Inc.
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Kimura Yoshinobu
Department Of Agricultural Science Faculty Of Agriculture Okayama University
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Sugimoto Hiroyuki
Yamada Apiculture Center
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Yamada Hidenori
Department Of Bioengineering Science Faculty Of Engineering Okayama University
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Yamada H
Institute For Bee Products And Health Science Yamada Apiculture Center Inc.
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TSUMURA Kazunori
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Kuroyanagi Masanori
Fac. Of Pharmaceutical Sciences At Kagawa Campus Tokushima Bunri Univ.
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Yamada Hisami
Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University
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OKIHARA Kiyoshi
Institute for Bee Products and Health Science, Yamada Apiculture Center, Inc.
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Okihara K
Institute For Bee Products And Health Science Yamada Apiculture Center Inc.
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Kimura Mariko
Fac. Of Food Culture Dep. Of Nutrition Kurashiki Sakuyo Univ.
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Tsumura K
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Okihara Kiyoshi
Institute For Bee Products And Health Science Yamada Apiculture Center Inc.
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Yamada Hideo
Institute For Bee Products And Health Science Yamada Apiculture Center Inc.
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Yamada Hideo
Yamada Apiculture Center Inc.
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