Structural Analysis of N-Glycans of Storage Glycoproteins in Soybean (Glycine max. L) Seed
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概要
- 論文の詳細を見る
The structures of N-linked sugar chains (N-glycans) of storage glycoproteins in soybean seeds have been identified. Eight pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the storage glycoproteins by reverse-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were first identified by two-dimensional PA-sugar chain mapping and ion-spray mass analysis, considering the results of sugar composition analysis or sequential exotlycosidase digestion. The deduced structures were further analyzed by ion-spray tandem mass spectrometry and 500 MHz ^1H-NMR spectrometry. The eight structures fell into two categories; the major class (96.6%) was a typical high mannose-type, the minor class was a xylose containing-type (Man_3Xyl_1GleNAc_2, Man_3Fuc_1Xyl_1GlcNAc_2; 3.4%).
- 社団法人日本農芸化学会の論文
- 1997-11-23
著者
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Kimura Yoshinobu
The International Center For Biotechnology Osaka University
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KIMURA Yoshinobu
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Kimura Y
Department Of Biological And Environmental Chemistry Faculty Of Agriculture Tottori University
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OHNO Akira
institute for Solid State Physics,University of Tokyo
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Ohno Akira
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Ohno A
Institute For Solid State Physics University Of Tokyo
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Kimura Yoshinobu
Department Of Agricultural Science Faculty Of Agriculture Okayama University
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TAKAGI Shigeaki
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Takagi S
Okayama Univ. Okayama Jpn
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Takagi Shigeaki
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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Takagi Shigeaki
Department of Agricultural Chemistry, Faculty of Agriculture Okayama University
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