Cloning and Sequence Analysis of a Protease-encoding Gene from the Marine Bacterium Alteromonas sp. Strain O-7

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概要

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• The gene (aprI) encoding alkaline serine protease (AprI; subtilase) from Alteromonas sp. strain O-7 was cloned and sequenced. The nucleotide sequence of aprI has been identified. The deduced amino acid sequence indicated that aprI codes for a precursor of 715 amino acids and the precursor is composed of four regions including a signal peptides an N-terminal pro-region, a mature protease region and a C-terminal extension region of 215 amino acids as previously described for aprII [H. Tsujibo et al., Gene, 136, 247-251(1993)]. The amino acid sequence of the mature AprI (AprI-M) showed high sequence homology with those of other class I subtilases. The C-terminal region was characterized by a repeat of 94 amino acids residues, which showed about 50% similarity with those of the C-terminal pro-region of several known proteases from Gram-negative bacteria.

• 社団法人日本農芸化学会の論文
• 1996-08-23

著者

• Kimura Yoshinobu

  The International Center For Biotechnology Osaka University

• Inamori Yoshihiko

  Department Of Microbiology Osaka University Of Pharmaceutical Sciences

• Kimura Y

  Department Of Biological And Environmental Chemistry Faculty Of Agriculture Tottori University

• MIYAMOTO Katsushiro

  Osaka University of Pharmaceutical Sciences

• INAMORI Yoshihiko

  Osaka University of Pharmaceutical Sciences

• IMADA Chiaki

  Institute of Microbial Chemistry

• OKAMI Yoshiro

  Institute of Microbial Chemistry

• TANAKA Kazumi

  Osaka University of Pharmaceutical Sciences

• KAIDZU Yoshihiko

  Osaka University of Pharmaceutical Sciences

• Okami Y

  Institute Of Microbial Chemistry

• Tsujiso Hiroshi

  Osaka University of Pharmaceutical Sciences

• Imada C

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Miyamoto K

  Central Research Laboratories Osaka University Of Pharmaceutical Sciences

• Inamori Yoshihiko

  Osaka College Of Pharmacy

• Miyamoto Katsushiro

  Osaka Univ. Of Pharmaceutical Sciences

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