Molecular Cloning of the Gene Encoding an Outer-Membrane-Associated β-N-Acetylglucosaminidase Involved in Chitin Degradation System of Alteromonas sp. Strain O-7
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概要
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The gene encoding β-N-acetylglucosaminidase (GlcNAcaseA) was cloned using PCR with degenerate oligonucleotide primers from the partial amino acid sequence of the enzyme. The gene encoded a polypeptide of 863 amino acids with a predicted molecular mass of 97 kDa. A characteristic signal peptide, which was present at the amino-terminus of the precursor protein, contained four amino acids (Ala-Gly-Cys-Ser) identical in sequence and location to the processing and modification sites of the outer membrane lipoprotein of Escherichia coli, indicating that the mature GlcNAcaseA is a lipoprotein the N-terminal cysteine residue of which would be modified by the fatty acid that anchors the protein in the membrane. The predicted amino acid sequence of GlcNAcaseA showed similarity to bacterial β-N-acetylglucosaminidases belonging to the family 20 glycosyl hydrolases.
- 社団法人日本農芸化学会の論文
- 2000-11-23
著者
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Inamori Yoshihiko
Department Of Microbiology Osaka University Of Pharmaceutical Sciences
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Miyamoto Junko
Osaka University Of Pharmaceutical Sciences
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TSUJIBO Hiroshi
Osaka University of Pharmaceutical Sciences
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MIYAMOTO Katsushiro
Osaka University of Pharmaceutical Sciences
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INAMORI Yoshihiko
Osaka University of Pharmaceutical Sciences
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KONDO Norihiko
Osaka University of Pharmaceutical Sciences
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BABA Nao
Osaka University of Pharmaceutical Sciences
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TSUJIBO Hiroshi
Department of Microbiology, Osaka University of Pharmaceutical Sciences
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Tsujibo H
Department Of Microbiology Osaka University Of Pharmaceutical Sciences
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Miyamoto K
Central Research Laboratories Osaka University Of Pharmaceutical Sciences
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Inamori Yoshihiko
Osaka College Of Pharmacy
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Miyamoto Katsushiro
Osaka Univ. Of Pharmaceutical Sciences
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