Isolation and Characterization of the Genes Encoding Two Metalloproteases (MprI and MprII) from a Marine Bacterium, Alteromonas sp. Strain O-7(Biochemistry & Molecular Biology)

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概要

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• An extracellular alkaline metalloprotease (MprI) from Alteromonas sp. strain O-7 was purified and characterized. The molecular mass of the purified enzyme was estimated to be 56 kDa by SDS-PAGE. The optimum pH and temperature were pH 10.0 and 60℃, respectively. The gene (mprI) encoding MprI was cloned and its nucleotide sequence was analyzed. The deduced amino acid sequence of MprI showed significant similarity to metalloproteases classified into the thermolysin family. Furthermore, sequence analysis showed that another metalloprotease (MprII)-encoding gene was located downstream from mprI. The deduced amino acid sequence of MprII showed high similarity to metalloproteases of the aminopeptidase family. Similar repeated C-terminal extensions were found in both MprI and MprII.

• 社団法人日本農芸化学会の論文
• 2002-02-23

著者

• Kimura Yoshinobu

  The International Center For Biotechnology Osaka University

• Inamori Yoshihiko

  Department Of Microbiology Osaka University Of Pharmaceutical Sciences

• Kimura Y

  Department Of Bioresource Science Faculty Of Agriculture Tottori University

• Kimura Y

  Department Of Biological And Environmental Chemistry Faculty Of Agriculture Tottori University

• Kimura Y

  Department Of Agricultural Science Faculty Of Agriculture Okayama University

• TSUJIBO Hiroshi

  Osaka University of Pharmaceutical Sciences

• MIYAMOTO Katsushiro

  Osaka University of Pharmaceutical Sciences

• INAMORI Yoshihiko

  Osaka University of Pharmaceutical Sciences

• TSUJIBO Hiroshi

  Department of Microbiology, Osaka University of Pharmaceutical Sciences

• NUKUI Eiji

  Osaka University of Pharmaceutical Sciences

• ITOH Hiroyuki

  Osaka University of Pharmaceutical Sciences

• KAIDZU Yoshihiko

  Osaka University of Pharmaceutical Sciences

• Tsujibo H

  Department Of Microbiology Osaka University Of Pharmaceutical Sciences

• Kimura Y

  Mukogawa Women's Univ. Nishinomiya Jpn

• Miyamoto K

  Central Research Laboratories Osaka University Of Pharmaceutical Sciences

• Kimura Y

  Department Of Biological And Environmental Chemistry Tottori University

• Inamori Yoshihiko

  Osaka College Of Pharmacy

• Miyamoto Katsushiro

  Osaka Univ. Of Pharmaceutical Sciences

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