Structural Elucidation of N-Linked Sugar Chains of Storage Glycoproteins in Mature Pea (Pisum sativum) Seeds by Ion-spray Tandem Mass Spectrometry (IS-MS/MS)

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概要

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• The structures of N-linked sugar chains of the storage glycoproteins in mature pea seeds have been estimated. Nine pyridylaminated (PA-) N-linked sugar chains were derived and purified from the hydrazinolysate of the storage glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were first identified by two-dimensional PA-sugar chain mapping, considering the data of sugar composition analysis or sequential exoglycosidase digestions. The deduced structures were further analyzed by IS-MS/MS analysis. Every relevant fragment ion derived from all PA-sugar chains could be assigned on the basis of deduced structures. The estimated nine structures fell into two categories; the first was a typical oligomannose type (Man_<8-3>GlcNAc_2; 77.7%) which can be hydrolyzed by endo-β-N-acetylglucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228-232 (1996)], the second was a xylose-containing type (Man_<4-3>Xyl_1GlcNAc_2, Man_3Fuc_1Xyl_1GlcNAc_2; 22.3%). Among these structures, Man_8GlcNAc_2 (19.7%), Man_6GlcNAc_2 (24.7%), and Man_3Fuc_1Xyl_1GlcNAc_2 (18.8%) were the dominant structures.

• 社団法人日本農芸化学会の論文
• 1996-11-23

著者

• Kimura Yoshinobu

  The International Center For Biotechnology Osaka University

• KIMURA Yoshinobu

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Kimura Y

  Department Of Biological And Environmental Chemistry Faculty Of Agriculture Tottori University

• OHNO Akira

  institute for Solid State Physics,University of Tokyo

• Ohno Akira

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Ohno A

  Institute For Solid State Physics University Of Tokyo

• Kimura Yoshinobu

  Department Of Agricultural Science Faculty Of Agriculture Okayama University

• TAKAGI Shigeaki

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Takagi S

  Okayama Univ. Okayama Jpn

• Takagi Shigeaki

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Takagi Shigeaki

  Department of Agricultural Chemistry, Faculty of Agriculture Okayama University

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