Changes in N-Linked Oligosaccharides during Seed Development of Ginkgo biloba
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概要
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Structural changes in N-linked oligosaccharides of glycoproteins during seed development of Ginkgo biloba have been explored to discover possible endogenous substrate (s) for the Ginko endo-β-N-acetylglucosaminidase (endo-GB ; Kimura, Y., et al. (1998) Biosci. Biotechnol. Biochem., 62,253-261), which should be involved in the production of high-mannose type free N-glycans. The structural analysis of the pyridylaminated oligosaccharides with a 2D sugar chain map, by ESIMS/MS spectroscopy, showed that all N-glycans expressed on glycoproteins through the developmental stage of the Ginkgo seeds have the xylose-containing type (GlcNAc_<2〜0>Man_3Xyl_1Fuc_<1〜0>GlcNAc_2) but no high-mannose type structure. Man3Xyl1Fuc1GlcNAc2,a typical plant complex type structure especially found in vacuolar glycoproteins, was a dominant structure through the seed development, while the amount of expression of GlcNAc_2Man_3Xyl_1Fuc_1GlcNAc_2 and GlcNAc_1Man_3Xyl_1Fuc_1GlcNAc_2 decreased as the seeds developed. The dominantly occurrence of xylose-containing type structures and the absence of the highmannose type structures on Ginkgo glycoproteins were also shown by lectin-blotting and immunoblotting of SDS-soluble glycoproteins extracted from the developing seeds at various developmental stages. Concerning the endogenous substrates for plant endo-β-N-acetylglucosaminidase, these results suggested that the endogenous substrates might be the dolicololigosaccharide intermediates or some glycopeptides with the high-mannose type N-glycan (s) derived from misfolded glycoproteins in the quality control system for newly synthesized glycoproteins.
- 社団法人日本農芸化学会の論文
- 2000-03-23
著者
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KIMURA Yoshinobu
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Kimura Yoshinobu
Department Of Agricultural Science Faculty Of Agriculture Okayama University
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MATSUO Sayuri
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Matsuo Sayuri
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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