A New Affinity Chromatography Using Yeast Invertase-Sepharose 4B for Purification of Plant Endo-β-N-acetylglucosaminidases

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概要

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• For the purification of plant endo-β-N-acetylglucosaminidase, in this report, we introduce a new affinity chromatography using the reduced and carboxymethylated yeast invertase (cm-YI) as a ligand. Two plant endo-β-N-acetylglucosaminidases (endo-LE from tomato fruits (Kimura, Y., et al. Biochim. Biophys. Acta 1381,27-36 (1998)) and endo-GB from Ginkgo biloba seeds (Kimura, Y., et al. Biosci. Biotechnol. Biochem., 62,253-261 (1998)) could completely bind to the high-mannose type N-glycans linked to the immobilized yeast invertase and the activities of both enzymes could be recovered by increasing the concentration of NaCl. By using this purification procedure with some other purification procedures, endo-LE could be purified 1,700-fold and endo-GB was purified to apparent homogeneity at 63 kDa as reported previously.

• 社団法人日本農芸化学会の論文
• 1999-05-23

著者

• KIMURA Yoshinobu

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Nakata Kengo

  Kagome Research Institute Kagome Co. Ltd.

• INOUE Hirotaka

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyushu University

• Kimura Yoshinobu

  Department Of Agricultural Science Faculty Of Agriculture Okayama University

• Harada Satoshi

  School Of Medicine And Center For Aids Research Kumamoto University

• Inoue Hirotaka

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Inoue Hirotaka

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• MATSUO Sayuri

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• HARADA Satoshi

  Kagome Research Institute, Kagome Co., Ltd.

• Matsuo Sayuri

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

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