Molecular Cloning and Gene Expression Analysis of Tomato Endo-β-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening
スポンサーリンク
概要
- 論文の詳細を見る
In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the high-mannose type N-glycans with the Manα1-2Manα1-3Manβ1-4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.
- 社団法人 日本農芸化学会の論文
- 2009-02-23
著者
-
Fujiyama Kazuhito
The International Center for Biotechnology, Osaka University
-
木村 吉伸
岡山大学農学部
-
木村 吉伸
岡山大学 農
-
INOUE Masami
Department of Hematology/Oncology, Osaka Medical Center, Research Institute for Maternal and Child H
-
KIMURA Yoshinobu
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
-
Fujiyama Kazuhito
International Center For Biotechnology Osaka Univ.
-
木村 吉伸
Dep. Of Biofunctional Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ.
-
MAEDA Megumi
Department of Hygiene, Kawasaki Medical School
-
Inoue Masami
Department Of Cell Engineering Faculty Of Engineering Toyama University
-
木村 吉伸
岡山大学
-
Maeda Megumi
Department Of Biofunctional Chemistry Division Of Bioscience Graduate School Of Natural Science And
-
Nakamura Kosuke
Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama Un
-
NAKANO Ryohei
Department of Plant Functions and Regulation, Graduate School of Natural Science and Technology, Oka
-
HOSOI Katsutoshi
Research Institute, Kagome Co., Ltd.
-
Kimura Yoshinobu
Department Of Agricultural Science Faculty Of Agriculture Okayama University
-
Kimura Yoshinobu
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
-
Fujiyama Kazuhito
The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn
-
Nakano Ryohei
Department Of Botany Graduate School Of Science Kyoto University
-
Nakamura Kosuke
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
-
Maeda M
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
-
Nakamura Kosuke
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
-
Hosoi Katsutoshi
Research Institute Kagome Co. Ltd.
-
Inoue Masami
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
-
Nakano Ryohei
Department Of Plant Functions And Regulation Graduate School Of Natural Science And Technology Okaya
関連論文
- The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants(PLANT BIOTECHNOLOGY)
- スギおよびヒノキ花粉アレルゲンに結合する糖タンパク質糖鎖の構造特性と免疫活性
- アレルゲン構成糖鎖の構造と活性 (特集 アレルゲンの分子医学) -- (アレルゲンの分子生物学的解析)
- Clinicopathological characteristics of erythroblast-rich RAEB and AML M6a in children
- The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants
- ヒト血漿α_1T糖蛋白質は高糖鎖含有アルブミンファミリータンパク質である : 物理化学・分析化学
- ヒマ種子カルス化に伴うアスパラギン結合型糖鎖の構造変化(英文)
- イチョウ種子中のendo-β-N-acetylglucosaminidase活性と貯蔵糖蛋白質糖鎖の構造解析 : 糖鎖工学
- エンドウ種子貯蔵糖蛋白質の全糖鎖構造 : 酵素
- エンドウ種子 Endo-β-N-acetylglucosaminidaseの酵素学的諸性質. : 糖鎖工学
- ラット小腸粘膜レチナール還元酵素活性の測定法の確立と酵素化学的性質
- ホウレンソウ クロロプラストからの色素-蛋白質複合体の分離・精製(生体高分子化学-多糖類, 核酸, 複合高分子-)
- Glycoproteins Secreted from Suspension-cultured Tobacco BY2 Cells have Distinct Glycan Structures from Intracellular Glycoproteins(Biochemistry & Molecular Biology)
- Identification of Putative Gene Encoded on ORF16 of the 81 kb Contig of Arabidopsis thaliana Chromosome III as α-Mannosidase
- Structures of N-Linked Oligosaccharides of Glycoproteins from Tobacco BY2 Suspension Cultured Cells
- 1330 Structures of N-linked Glycoproteins from Tobacco BY2 Suspension Cultured Cells
- 293 遊離型糖鎖を用いたCryj1構成糖鎖のIgEエピトープ活性に関する検討
- 291 遊離型糖鎖によるCryj1特異的T細胞応答の制御
- In Planta Production of Immunogenic Poliovirus Peptide Using Tobacco Mosaic Virus-Based Vector System(PLANT BIOTECHNOLOGY)
- Human N-Acetylglucosaminyltransferase I. : Expression in Escherichia coli as a Soluble Enzyme, and Application as an Immobilized Enzyme for the Chemoenzymatic Synthesis of N-Linked Oligosaccharides
- Chronic Graft-Versus-Host Desease in Children and Adolescents After Bone Marrow Transplantation From HLA-Matched Donors
- ウズラ反転小腸によるホウレンソウ葉カロテノイドの吸収とレチノイド生成及び血中カテロイドの卵黄への移行機作について
- Glycoform Analysis of N-Glycans Linked to Glycoproteins Expressed in Rice Culture Cells : Predominant Occurrence of Complex Type N-Glycans
- 206 マンノース結合レクチンのスギ花粉症における役割(自然免疫, 第55回日本アレルギー学会秋季学術大会)
- 5 花粉アレルゲンに結合するアスパラギン結合型糖鎖の構造特性と免疫活性
- 植物複合型遊離N-グリカンが細胞壁に局在する可能性(英文)
- 植物細胞内に存在する遊離N-グリカンの構造,機能および生成
- タケノコ(ハチク)中に存在する遊離N-グリカンの構造解析(英文)
- 植物糖蛋白質糖鎖の構造と機能および植物細胞由来のNグリカン遊離酵素に関する研究
- 植物糖蛋白質糖鎖の構造と機能および植物細胞由来のN-グリカン遊離酵素
- Anesthetic management of two cases of Beckwith-Wiedemann syndrome
- Cloning and characterization of cytidine monophosphate-3-deoxy-D-manno-octulosonate synthetase from Arabidopsis thaliana(PLANT BIOTECHNOLOGY)
- N-Linked Glycan Structures of a Mouse Monoclonal Antibody Produced from Tobacco BY2 Suspension-Cultured Cells(CELL AND TISSUE ENGINEERING)
- Characterization of Almond α-Mannosidase and Its Application for Structure Analysis of Sugar Chain(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Effect of αl, 2-Mannosidic Linkage Located in α1, 3-Branch of Man6GlcNAc2 Oligosaccharide on Enzyme Activity of Recombinant Human Man_9-Mannosidase Produced in Escherichia coli
- 自然科学の楽しさを伝えたい : 岡山大学サイエンス・スクールの試み
- N-Linked Sugar Chains of 350-kDa Royal Jelly Glycoprotein
- Enhancement of Recombinant Soluble Dengue Virus 2 Envelope Domain III Protein Production in Escherichia coli trxB and gor Double Mutant(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- 植物糖蛋白質糖鎖のプロセシング-糖転移酵素の関与する2つの推定経路(今日の話題)
- Intracellular and extracellular free N-glycans produced by plant cells: occurrence of unusual plant complex-type free N-glycans in extracellular spaces
- Molecular Cloning and Gene Expression Analysis of Tomato Endo-β-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening
- Glycoform Analysis of Japanese Cypress Pollen Allergen, Cha o 1: A Comparison of the Glycoforms of Cedar and Cypress Pollen Allergens
- Tumor Antigen Occurs in N-Glycan of Royal Jelly Glycoproteins : Honeybee Cells Synthesize T-Antigen Unit in N-Glycan Moiety
- Occurrence of Lewis a Epitope in N-Glycans of a Glycoallergen, Jun a 1, from Mountain Cedar (Juniperus ashei) Pollen
- Structural Features of N-Glycans Linked to Glycoproteins from Oil Palm Pollen, an Allergenic Pollen(Biochemistry & Molecular Biology)
- Purification and Characterization of 31-kDa Palm Pollen Glycoprotein (Ela g Bd 31 K), Which is Recognized by IgE from Palm Pollinosis Patients(Biochemistry & Molecular Biology)
- Zeaxanthine分子集合体の分光学的挙動とその形態
- ホウレンソウ葉カロテノイドがヒト白血病細胞株の分化に及ぼす影響
- β一Carotene及びホウレンソウカロテノイドのラットにおける吸収と蓄積
- 緑葉カロテノイドのニワトリ卵黄への選択的移行とその機構
- 水系におけるCarotenoid集合体のらせん構造 : LuteinとZeaxanthin(生体高分子化学-脂質-)
- Effects of Glucose and Glycine on the Biodegradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB / V) and the Proliferation of PHB / V-Degrading Microorganisms in Soil Suspension
- Estimation of the Number of Polyhydroxyalkanoate (PHA)-Degraders in Soil and Isolation of Degraders Based on the Method of Most Probable Number (MPN) Using PHA-Film
- Predominant Occurrence of Truncated Complex Type N-Glycans among Glycoproteins in Mature Red Tomato
- N-linked glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells
- Changes in Structural Features of Free N-Glycan and Endoglycosidase Activity during Tomato Fruit Ripening
- 生化学・栄養 大豆種子貯蔵糖たん白質に結合する糖鎖抗原とアレルギー疾患との相関解析
- Isolated Ricin B-Chain-mediated Apoptosis in U937 Cells
- Effects of Amino Acids on the Amidation of Polyaromatic Carboxylic Acids by Bacillus cereus(Microbiology & Fermentation Technology)
- Autologous Hematopoietic Stem Cell Transplantation for 3 Patients with Severe Juvenile Rheumatoid Arthritis
- Salt-Adaptation of Tobacco BY2 Cells Induces Change in Glycoform of N-Glycans: Enhancement of Exo- and Endo-Glycosidase Activities by Salt-Adaptation
- Evidence for new beta 1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera
- 350-kDa Royal Jelly Glycoprotein (Apisin), Which Stimulates Proliferation of Human Monocytes, Bears the β1-3Galactosylated N-Glycan : Analysis of the N-Glycosylation Site(Biochemistry & Molecular Biology)
- First Evidence for Occurrence of Galβ1-3GlcNAcβ1-4Man Unit in N-Glycans of Insect Glycoprotein : β1-3Gal and β1-4GlcNAc Transferases Are Involved in N-Glycan Processing of Royal Jelly Glycoproteins(Biochemistry & Molecular Biology)
- Antiviral Activity of a Hot Water Extract of Black Soybean against a Human Respiratory Illness Virus(Food & Nutrition Science)
- Occurrence of GalNAcβ1-4GlcNAc Unit in N-Glycan of Royal Jelly Glycoprotein(Biochemistry & Molecular Biology)
- Structural Features of N-Glycans Linked to Royal Jelly Glycoproteins : Structures of high-mannose type, hybrid type, and biantennary type glycans
- A New Affinity Chromatography Using Yeast Invertase-Sepharose 4B for Purification of Plant Endo-β-N-acetylglucosaminidases
- N-Linked Sugar Chain of 55-kDa Royal Jelly Glycoprotein
- Circannual Changes in Corticosterone Plasma Levels and Binding of Corticosterone to Plasma in Bufo japonicus formosus(Endocrinology)
- Structural Analysis of N-Linked Oligosaccharide of Mitogenic Lectin-B from the Roots of Pokeweed (Phytolacca americana)
- CHRONIC ACTIVE EPSTEIN-BARR VIRUS INFECTION AND LYMPHOPROLIFERATIVE DISEASES
- CD34^+ progenitor cell transplantation from two HLA-mismatched healthy fathers to two infants with severe aplastic anemia
- Structural Analysis of N-Glycans of Storage Glycoproteins in Soybean (Glycine max. L) Seed
- Panose Production by a Mutant of Streptomyces hygroscopicus subsp.limoneus
- Structural Elucidation of N-Linked Sugar Chains of Storage Glycoproteins in Mature Pea (Pisum sativum) Seeds by Ion-spray Tandem Mass Spectrometry (IS-MS/MS)
- Occurrence of Free N-Glycans in Pea (Pisum sativum. L) Seedlings
- Encouraging Results of Low-Dose Etoposide in the Treatment of Early-Onset Hemophagocytic Syndrome Following Allogeneic Hematopoietic Stem Cell Transplantation
- ラット反転小腸におけるβ-カロテンの吸収と代謝挙動
- Growth deceleration in a girl treated with imatinib
- Upregulated Expression of Ibal Molecules in the Central Nervous System of Mice in Response to Neurovirulent Influenza A Virus Infection
- Double-Knockout of Putative Endo-β-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana: Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N′-Acetylchitobiosyl Unit
- Production and Characterization of Active Soluble Human β1, 4-Galactosyltransferase in Escherichia coli as a Useful Catalyst in Synthesis of the Gal β1⟶4 GlcNAc Linkage
- Purification and Some Chemical Properties of 30 kDa Ginkgo biloba Glycoprotein, which Reacts with Antiserum Against β1→2 Xylose-Containing N-Glycans
- Changes in N-Linked Oligosaccharides during Seed Development of Ginkgo biloba
- Identification of a Royal Jelly Glycoprotein That Carries Unique Complex-Type N-Glycans Harboring the T-Antigen (Galβ1-3GalNAc) Unit
- Salt-Adaptation of Tobacco BY2 Cells Induces Change in Glycoform of N-Glycans : Enhancement of Exo- and Endo-Glycosidase Activities by Salt-Adaptation
- Evidence for new beta 1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera
- Glycoform Analysis of Japanese Cypress Pollen Allergen, Cha o 1 : A Comparison of the Glycoforms of Cedar and Cypress Pollen Allergens
- Molecular Cloning and Expression of Two Novel β-N-Acetylglucosaminidases from Silkworm Bombyx mori
- Molecular Cloning and Gene Expression Analysis of Tomato Endo-β-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening
- Predominant Occurrence of Truncated Complex Type N-Glycans among Glycoproteins in Mature Red Tomato
- Changes in Structural Features of Free N-Glycan and Endoglycosidase Activity during Tomato Fruit Ripening
- Purification and Characterization of a CM-Glycinin Digesting Protease from Soybean Seeds
- α-Mannosidase Involved in Turnover of Plant Complex Type N-Glycans in Tomato (Lycopersicum esculentum) Fruits
- Molecular characterization of plant acidic α-mannosidase, a member of glycosylhydrolase family 38, involved in the turnover of N-glycans during tomato fruit ripening
- Molecular identification and characterization of an acidic peptide : N-glycanase from tomato (Lycopersicum esculentum) fruits
- Intracellular and extracellular free N-glycans produced by plant cells : occurrence of unusual plant complex-type free N-glycans in extracellular spaces
- 遊離型胆汁酸塩によって分散されたルテインの分光学的挙動と形態
- Double-Knockout of Putative Endo-β-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana : Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N'-Acetylchitobiosyl Unit