植物複合型遊離N-グリカンが細胞壁に局在する可能性(英文)

概要

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In this report, we bring up the possibility that complex type free N-glycans may localize in cell wall fraction of developing seeds (Ginkgo biloba seeds). Several free N-glycans extracted by mild acid hydrolysis of cell wall fraction were coupled with 2-aminopyridine and purified by gel filtration, size-fractionation HPLC, and reversed-phase HPLC. The structures of the pyridylaminated free N-glycans were identified by two-dimensional sugar chain mapping, α-1,2-mannosidase digestions, and ionspray tandem mass spectrometry. The structural analyses showed that highmannose type free N-glycans having one GlcNAc residue (Man8-5GlcNAc1) and plant complex type free N-glycans having the N-acetyl chitobiose unit also occur in the call wall fraction of the developing Ginkgo seeds. However, quantitative analysis of such free N-glycans showed that the plant complex type free glycans found in small amounts(～3%) in the cytosolic fraction accounted for nearly 40% of total free N-glycans, This observation suggested that the complex type free N-glycans might occur and localized in the cell wall fraction.
岡山大学の論文
2001-02-01