Double-Knockout of Putative Endo-β-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana: Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N′-Acetylchitobiosyl Unit
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概要
- 論文の詳細を見る
Endo-β-N-acetylglucosaminidase (ENGase) is involved in the production of high-mannose type free N-glycans during plant development and fruit maturation. In a previous study (K. Nakamura et al. Biosci. Biotechnol. Biochem., 73, 461–464 (2009)), we identified the tomato ENGase gene and found that gene expression remained relatively constant. In the present study, we constructed an Arabidopsis thaliana mutant in which the expression of two putative ENGase genes was suppressed. The mutant showed no ENGase activity, but produced high-mannose type free N-glycans carrying the N,N′-acetylchitobiosyl unit that is produced by peptide:N-glycanase, indicating that both these genes encode Arabidopsis ENGase.
- 社団法人 日本農芸化学会の論文
著者
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Fujiyama Kazuhito
International Center for Biotechnology, Osaka University
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木村 吉伸
岡山大学農学部
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木村 吉伸
岡山大学 農
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INOUE Masami
Department of Hematology/Oncology, Osaka Medical Center, Research Institute for Maternal and Child H
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KIMURA Yoshinobu
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Fujiyama Kazuhito
International Center For Biotechnology Osaka Univ.
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木村 吉伸
Dep. Of Biofunctional Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ.
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MAEDA Megumi
Department of Hygiene, Kawasaki Medical School
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Inoue Masami
Department Of Cell Engineering Faculty Of Engineering Toyama University
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木村 吉伸
岡山大学
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Maeda Megumi
Department Of Biofunctional Chemistry Division Of Bioscience Graduate School Of Natural Science And
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Kimura Yoshinobu
Department Of Agricultural Science Faculty Of Agriculture Okayama University
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TAKEOKA Yuki
Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama Un
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