Occurrence of Lewis a Epitope in N-Glycans of a Glycoallergen, Jun a 1, from Mountain Cedar (Juniperus ashei) Pollen
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概要
- 論文の詳細を見る
We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a 1-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a 1-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (Galβ1–3(Fucα1–4)GlcNAcβ1–) occurs in the N-glycans of the pollen allergens.
- 社団法人 日本農芸化学会の論文
- 2005-01-23
著者
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木村 吉伸
岡山大学農学部
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木村 吉伸
岡山大学 農
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OKANO Mitsuhiro
Department of Otolaryngology/Head & Neck Surgery, Okayama University Medical School and Graduate Sch
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KIMURA Yoshinobu
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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木村 吉伸
Dep. Of Biofunctional Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ.
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MAEDA Megumi
Division of Bioscience, Department of Biofunctional Chemistry, Graduate School of Natural Science an
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木村 吉伸
岡山大学
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Kimura M
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Maeda Megumi
Division Of Bioscience Department Of Biofunctional Chemistry Graduate School Of Natural Science And
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YOKOYAMA Minehiko
Meiji Co.
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KINO Kosuke
Meiji Co.
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KAMAMOTO Maiko
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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Okano Mitsuhiro
Department Of Otolaryngology-head And Neck Surgery Okayama University Graduate School Of Medicine An
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Okano Mitsuhiro
Department Of Mechanical Engineering Kansai University
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Kimura Yoshinobu
Department Of Agricultural Science Faculty Of Agriculture Okayama University
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Kawabe Masato
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology (tuat)
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Kuroyanagi Masanori
Fac. Of Pharmaceutical Sciences At Kagawa Campus Tokushima Bunri Univ.
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Maeda Megumi
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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Kimura Yoshinobu
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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Kimura Mariko
Fac. Of Food Culture Dep. Of Nutrition Kurashiki Sakuyo Univ.
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Maeda M
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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MAEDA Megumi
Division of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University
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