Primary Structures of N-Linked Oligosaccharides of Momordin-a, a Ribosome-inactivating Protein from Momordica charantia Seeds(Biological Chemistry)

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概要

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• The structures of thee N-linked oligosaccharides of momordin-a, which is a ribosome-inactivating protein from the seeds of Momordica charantia, were analyzed. First, the N-linked oligosaccharides of this glycoprotein were liberated by hydrazinolysis. After N-acetylation, the reducing ends of the oligosaccharides were coupled with 2-aminopyridine and the pyridylamino (PA-) derivatives were purified by gel filtration and high performance liquid chromatography (HPLS) on an ODS-silica column. Three kinds of oligosaccharide fractions were separated by HPLS. The structure of each oligosaccharide isolated was analyzed by a combination of sugar component analysis, exoglycosidase digestion, another kind of HPLC using an amide-silica column, and 500-MHz ^1H NMR spectroscopy. The structures of two main oligosaccharides were established to be: [chemical formula] and [chemical formula] These two oligosaccharides were the first examples having xylose (or fucose) but no α-mannosyl linkage among the N-linked oligosaccharides of glycoproteins from both animal and plant origins.

• 社団法人日本農芸化学会の論文
• 1991-08-23

著者

• FUNATSU Gunki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• KIMURA Yoshinobu

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Kimura Y

  Department Of Bioresource Science Faculty Of Agriculture Tottori University

• Nakajima S

  Graduate School Of Natural Science And Technology Okayama University

• Nakajima Shuhei

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Nakajima Shuhei

  Department Of Agricultural Science Okayama University

• MINAMI Yuji

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Kimura Yoshinobu

  Department Of Agricultural Science Faculty Of Agriculture Okayama University

• Minami Yuji

  Laboratory Of Protein Chemistry And Engineering Faculty Of Agriculture Kyushu University

• Funatsu G

  Kyushu Univ. Fukuoka Jpn

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• TAKAGI Shigeaki

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• Takagi S

  Okayama Univ. Okayama Jpn

• Takagi Shigeaki

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Minami Yuji

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• TOKUDA Toshihiko

  Department of Agricultural Sciences, Faculty of Agriculture, Okayama University

• Tokuda Toshihiko

  Department Of Agricultural Sciences Faculty Of Agriculture Okayama University

• Takagi Shigeaki

  Department of Agricultural Chemistry, Faculty of Agriculture Okayama University

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