Denaturation of Ricin D in Frozen Aqueous Solutions(Biological Chemistry)
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概要
- 論文の詳細を見る
Ricin D was denatured in frozen acidic solutions accompanied by the insolubilization. Kinetic studies indicated tthat the insolubilization of ricin D occurred during the incubation of the frozen solution at -51℃〜-5℃, but not in the freezing process at -80℃ or the thawing process. Such an insolubilization of ricin D in the frozen system was accelerated by lowering the pH below pH 4.0, and the optimum temperature was found at -29℃. The rate of the insolubilization of ricin D decreased on the addition of AaCl or on elevation of the temperature from -29℃ to -5℃, or on increasing the protein concentration above 0.15%. The freeze-denaturation of ricinD was prevented by saccharides, and lactose, with a higher affinity for ricin D than galactose, suppressed the insolubilization of ricin D more effectively than galactose. A slight suppressive effect of lactose was found for insolubilization of a derivative of ricin D in which the saccharide-binding ability of the low affinity saccharide-binding site was destroyed by N-bromosuccinimide-oxidation. It is suggested that the denaturation of ricin D in the frozen acidic solution is principally due to the concentration of protons and solutes into licuid regions surrounded by ice crystals. Spectroscopic data also suggest that the protonation of fhe ionizable group(s) with pk_a=4〜5 may be closely associated with the denaturation of ricin D in the frozen state below pH 4.0.
- 社団法人日本農芸化学会の論文
- 1988-10-23
著者
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Hatakeyama T
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Hatakeyama Tomomitsu
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
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Hatakeyama Tomomitsu
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University:(present Address)dept. Of Applie
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YAMASAKI Nobuyuki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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FUNATSU Gunki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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Tomomitsu Hatakeyama
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Yamasaki Nobuyuki
Institute Of Biophysics Faculty Of Agriculture Kyushu University
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Yamasaki N
Kyushu Univ. Fukuoka Jpn
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Funatsu Gunki
Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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