Resolution and Characterization of Tryptophyl Fluorescence of Hen Egg-White Lysozyme by Quenching-and Time-Resolved Spectroscopy

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概要

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• The fluorescence spectral distributions of four tryptophan residues of hen egg-white lysozyme were analyzed using time-resolved and quenching-resolved fluorescence spectroscopy. Trp62 and Trp108 gave the fluorescence maxima at 352 nm and 342 nm, respectively. The fluorescence of Trp28 and Trp111 occurred only at 300-360 nm and they were observed as an unresolved emission band with a maximum and shoulder at 320 nm and 330 nm. The fluorescence quenching and decay parameters of each tryptophan residue reconfirmed that Trp62 was fully exposed to the solvent but Trp108 was sealed in the cage of the peptide chains and furthermore showed that Trp28 and Trp111 are under the influence of the larger fluctuational motion at the hydrophobic matrix box. The fluorescence responses of each tryptophan residue to the lysozyme-ligand interaction suggested that the internal fluctuation was reduced by the binding of ligand to give a distorted conformation to the hydrophobic matrix box region.

• 社団法人日本農芸化学会の論文
• 1999-02-23

著者

• YAMASAKI Nobuyuki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• IMOTO Taiji

  Faculty of Biotechnology and Life Science, Sojo University

• Imoto T

  Faculty Of Biotechnology And Life Science Sojo University

• Imoto T

  Kyushu Univ. Fukuoka Jpn

• Imoto Taiji

  Graduate School Of Pharmaceutical Sciences

• NISHIMOTO Etsuko

  Division of Material Chemistry, Kyushu National Industrial Research Institute

• YAMASHITA Shoji

  Institute of Biophysics, Faculty of Agriculture, Kyushu University

• Yamashita Shoji

  Faculty Of Agriculture Graduate School Of Kyushu Univ

• Yamashita Shoji

  Institute Of Biophysics Faculty Of Agriculture Kyushu University

• Nishimoto E

  Kurume Univ. School Of Medicine Fukuoka Jpn

• Imoto Taiji

  Faculty Of Biotechnology And Life Science Sojo University

• Imoto Taiji

  九州大学 薬学研究院

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur

• Yamasaki N

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Yamashita S

  Institute Of Biophysics Faculty Of Agriculture Graduate School Of Kyushu University

• Yamashita Shoji

  Institute Of Biophysics Faculty Of Agriculture Graduate School Of Kyushu University

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Yamasaki Nobuyuki

  Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University

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