Cytotoxicity Induced by Erythrina variegata Serine Proteinase Inhibitors in Tumor Hematopoietic Stem Cell Lines
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概要
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Based on the soluble MTT tetrazolium/formazan assay, we evaluated the cytotoxicity of Erythrina variegata proteinase inhibitors in some tumor hematopoietic stem cell lines. Among the proteinase inhibitors, EBI, which belongs to the Bowman-Birk family of inhibitors, was cytotoxic in relatively differentiated cells such as Molt4 and Jurkat derived from acute T lymphoblastic leukemia (T-ALL) cells specifically, but ETIa and ECI, which are classified into Kunitz family inhibitors, did not. It was suggested that the differences in the cytotoxicity might be due to the molecular size of the inhibitors. The succinylation of lysine residue (s) of EBI led to about 50% loss of the trypsin inhibitory activity as compared with the authentic EBI. When Molt4 cells were incubated with this derivative, no significant cytotoxicity was observed. This suggests that the proteinase inhibitory activity might be involved in the cytotoxicity in human tumor cell lines.
- 1998-06-23
著者
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ITOH Kyogo
Departments of Immunology, Kurume University School of Medicine
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Ohba Hideki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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YAMASAKI Nobuyuki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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KIMURA Makoto
Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University
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Itoh K
Univ. Tokyo Tokyo Jpn
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Itoh Kyogo
Department Of Immunology
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OHBA Hideki
Kyushu National Industrial Research Institute
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NISHIKAWA Masaaki
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
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MORIWAKI Sawako
Department of Applied Biological Sciences, Saga University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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Yamasaki N
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Ohba H
Kyushu National Industrial Res. Inst. Saga Jpn
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Morikawa S
Fukui Univ. Fukui Jpn
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Nishikawa M
Division Of Biology Department Of Earth System Science Faculty Of Science Fukuoka University
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Kimura Makoto
Lab. Of Structural Biology Graduate School Of Systems Life Sciences Kyushu Univ.
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Moriwaki Sawako
Department Of Applied Biological Sciences Saga University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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Kimura Makoto
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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Nishikawa Masaaki
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University
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Kimura Makoto
Laboratory Of Biochemistry Department Of Agricultural Chemistry Faculty And Graduate School Of Agric
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KIMURA Makoto
Laboratory for Remediation Research, Plant Science Center, RIKEN
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