Amino Acid Sequence and Carbohydrate-binding Analysis of the N-acetyl-D-galactosamine-specific C-Type Lectin, CEL-I, from the Holothuroidea, Cucumaria echinata(Biochemistry & Molecular Biology)

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概要

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• CEL-I is one of the Ca^<2+> -dependent lectins that has been isolated from the sea cucumber, Cucumaria echinata. This protein is composed of two identical subunits held by a single disulfide bond. The complete amino acid sequence of CEL-I was determined by sequencing the peptides produced by proteolytic fragmentation of S-pyridylethylated CEL-I. A subunit of CEL-I is composed of 140 amino acid residues. Two intrachain (Cys3-Cys14 and Cys31-Cys135) and one interchain (Cys36) disulfide bonds were also identified from an analysis of the cystine-containing peptides obtained from the intact protein. The similarity between the sequence of CEL-I and that of other C-type lectins was low, while the C-terminal region, including the putative Ca^<2+> and carbohydrate-binding sites, was relatively well conserved. When the carbohydrate-binding activity was examined by a solid-phase microplate assay, CEL-I showed much higher affinity for N-acetyl-D-galactosamine than for other galactose-related carbohydrates. The association constant of CEL-I for p-nitrophenyl N-acetyl-β-D-galactosaminide (NP-GalNAc) was determined to be 2.3 × 10^4 M^<-1>, and the maximum number of bound NP-GalNAc was estimated to be 1.6 by an equilibrium dialysis experiment.

• 社団法人日本農芸化学会の論文
• 2002-01-23

著者

• Hirawama Toshiya

  Department Of Bacteriology Institute Of Tropical Medicine Nagasaki University

• AOYAGI Haruhiko

  Kyushu Nutrition Welfare University

• HATAKEYAMA Tomomitsu

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• Hatakeyama T

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Hatakeyama T

  Nagasaki Univ. Nagasaki Jpn

• Hatakeyama Tomomitsu

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University:(present Address)dept. Of Applie

• YAMASAKI Nobuyuki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Aoyagi H

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Aoyagi H

  Kyushu Nutrition Welfare University

• AOYAGI Haruhiko

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• Tomomitsu Hatakeyama

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Sugawara Hajime

  Metabolic Function Research Group Riken Plant Science Center

• SHIBA Kouhei

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• MATSUO Noriaki

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• NISHINOHARA Shoichi

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur

• Hatakeyama Tomomitsu

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University

• Aoyagi Haruhiko

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Aoyagi Haruhiko

  Faculty Of Pharmaceutical Science:faculty Of Science:kyushu University

• Yamasaki N

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Hara Toshiaki

  Department Of Chemistry Faculty Of Science And Engineering Saga University

• Aoyagi H

  Faculty Of Engineering Nagasaki University

• Matsuo Noriaki

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Shiba Kouhei

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Nishinohara Shoichi

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr

• Yamasaki Nobuyuki

  Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University

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