Molecular Mechanism for Pore-formation in Lipid Membranes by the Hemolytic Lectin CEL-III from Marine Invertebrate Cucumaria echinata

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概要

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• The pore-forming activity of CEL-III, a Gal/GalNAc specific lectin from the Holothuroidea Cucumaria echinata, was examined using artificial lipid membranes as a model system of erythrocyte membrane. The carboxyfluorescein (CF)-leakage studies clearly indicated that CEL-III induced the formation of pores in the dipalmitoyl phosphatidyl choline (DPPC)-lactosyl ceramide (LacCer) liposomes effectively but not in the DPPC-glucosyl ceramide (GlcCer) liposomes or DPPC liposomes. Such a leakage of CF was strongly inhibited by lactose, a potent inhibitor of CEL-III, suggesting that the leakage is mediated through the specific binding of CEL-III to the carbohydrate chains on the surface of the liposomes. The leakage of CF from the DPPC-lactosyl ceramide liposomes was pH-dependent, and it increased with increasing pH. The immunoblotting analysis and circular dichroism data indicated that upon interaction with liposomes, CEL-III associated to from an oligomer concomitantly with a marked conformational change. Furthermore, channel measurements showed that CEL-III has an ability to from small ion channels in the planar lipid bilayers consisting of diphytanoylphosphatidylcholine and human globoside (Gb_4Cer)/LacCer.

• 社団法人日本農芸化学会の論文
• 1999-07-23

著者

• HATAKEYAMA Tomomitsu

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• YAMASAKI Nobuyuki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• JELOKHANI-NIARAKI Masood

  Department of Chemistry, Wilfrid Laurier University

• KONDO Michio

  Department of Chemistry, Faculty of Science and Engineering, Saga University

• EHARA Tsuguhisa

  Department of Physiology, Saga Medical School

• Yamasaki Nobuyuki

  Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur

• Jelokhani‐niaraki M

  Department Of Chemistry Wilfrid Laurier University

• Jelokhani-niaraki Masod

  Department Of Chemistry Faculty Of Science Wilfrid Laurier University

• Hatakeyama Tomomitsu

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University

• KOURIKI-NAGATOMO Haruna

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• Kondo M

  Department Of Health And Welfare Nishikyushu University

• Ehara Tsuyuko

  Department Of Physiology Saga Medical School

• Kouriki-nagatomo Haruna

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Ehara Tsuguhisa

  Department Of Physiology Faculty Of Medicine Kyushu University

• Kondo Michio

  Faculty Of Science And Engineering Saga University

• Kondo Michio

  Department Of Chemistry Faculty Of Science And Ehgineering Saga University

• Yamasaki Nobuyuki

  Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University

• Jelokhani-Niaraki Masood

  Department of Chemistry, Faculty of Science, Wilfrid Laurier University

• Jelokhani-Niaraki Masood

  Department of Chemistry, Faculty of Science and Engineering, Saga University

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