CEL-I, an N-acetylgalactosamine (GalNAc)-specific C-type lectin, induces nitric oxide production in RAW264.7 mouse macrophage cell line.
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概要
- 論文の詳細を見る
We found that CEL-I, a GalNAc-specific C-type lectin isolated from the marine invertebrate Holothuroidea (Cucumaria echinata), induces inducible nitric oxide synthase (iNOS) expression and NO production in RAW264.7 cells. The NO production was inhibited by an iNOS inhibitor, L-NAME, but was not by a lipopolysaccharide (LPS) inhibitor, polymyxin B. In the presence of 0.1-M GalNAc, increased NO production by CEL-I-treated RAW264.7 cells was observed rather than the inhibition. Bovine serum albumin (BSA) significantly inhibited the CEL-I-induced NO production as well as the binding of FITC-labelled CEL-I on RAW264.7 cells. Three MAP kinase inhibitors (specific to extra-cellular regulated kinase, c-jun NH(2)-terminal kinase and p38 MAP kinase) inhibited CEL-I-induced NO production with different extents. Heat-treatment of CEL-I resulted in a decreased activity of CEL-I depending on the temperature. These results suggest that CEL-I induces NO production in RAW264.7 cells through the protein-cell interaction rather than the binding to the specific carbohydrate chains on the cell surface.
- Japanese Biochemical Society,日本生化学会の論文
- 2009-08-01
著者
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Oda Tatsuya
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Oda Tatsuya
Div. Of Biochemistry Fac. Of Fisheries Nagasaki Univ.
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Oda T
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Hirawama Toshiya
Department Of Bacteriology Institute Of Tropical Medicine Nagasaki University
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HATAKEYAMA Tomomitsu
Department of Applied Chemistry, Faculty of Engineering, Nagasaki University
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YAMAGUCHI Kenichi
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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Hatakeyama T
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Hatakeyama T
Nagasaki Univ. Nagasaki Jpn
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Hatakeyama Tomomitsu
Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University:(present Address)dept. Of Applie
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Yamaguchi Kenichi
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Yamanishi Tomohiro
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Tomomitsu Hatakeyama
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Tatsuya Oda
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Oda T
Nagasaki Univ. Nagasaki Jpn
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Oda T
New Industry Creation Hatchery Center Tohoku University
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Okamoto Tarou
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Hatakeyama Tomomitsu
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Oda T
Faculty Of Fisheries Nagasaki University
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Hara Toshiaki
Department Of Chemistry Faculty Of Science And Engineering Saga University
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Yamaguchi Kenichi
New Industry Creation Hatchery Center Tohoku University
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YAMAGUCHI Kenichi
Division of Allergy and Rheumatology, St. Luke's International Hospital
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