Effects of Glutathione-related Compounds on Increased Caspase-3 and Caspase-6-like Activities in Ricin-treated U937 Cells
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概要
- 論文の詳細を見る
Both caspase-3 and -6-like activities increased in the cytosolic extract from ricin-treated U937 cells that were inhibited by glutathione disulfide (GSSG) in a dose-dependent manner, but reduced glutathione (GSH) had no effect. Interestingly, caspase-6 like activity was more sensitive to GSSG than caspase-3 like activity. The IC_<50> of GSSG against caspase-3 and caspase-6-like activities were estimated to be 2.8 mM and 0.8 mM, respectively. Cystine but not cysteine also showed similar inhibitory effect on caspase-3-like activity. The inhibitory effect of GSSG on these caspase-like activities was prevented by the addition of DTT to the assay mixture. These results suggest that an intact disulfide portion of GSSG is required for the effective inhibition of caspase activity.
- 社団法人日本農芸化学会の論文
- 2000-01-23
著者
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Oda Tatsuya
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Oda Tatsuya
Div. Of Biochemistry Fac. Of Fisheries Nagasaki Univ.
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Oda T
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Tatsuya Oda
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Oda T
Nagasaki Univ. Nagasaki Jpn
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Oda T
New Industry Creation Hatchery Center Tohoku University
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MURAMATSU Tsuyoshi
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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Okamoto Tarou
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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KOMATSU Nobukazu
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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SADAKATA Noriko
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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Muramatsu Tsuyoshi
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Muramatsu Tsuyoshi
Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo
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Muramatsu T
Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo
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Sadakata Noriko
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Komatsu Nobukazu
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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