Isolation and Characterization of a Mutant Strain of Chattonella marina with Decreased Production of Superoxide Anion

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概要

• 論文の詳細を見る

• Chattonella marina is well known as the causative organism of red tide, and is highly toxic to fish. However, the toxic mechanism of C. marina has not been established. Recent studies demonstrated that C. marina generates reactive oxygen species (ROS) such as O^-_2 and H_2O_2. In this study, we attempted to establish mutant strains of C. marina using a chemical mutagen, ethyl methane sulfonate (EMS). After 48 h of treatment with 1 mg/ ml of EMS at 26℃, several viable cell were cloned, while more than 90% of C. marina cells died under these conditions. Among the strains isolated, one strain (mutant C) was significantly decreased in the production of O^-_2,but its growth rate was indistinguishable from the parental C. marina. Furthermore, superoxide dismutase (SOD) had almost no effect on the proliferation of mutant C, while SOD suppressed the growth of parental C. marina. In accordance with the lower level of O^-_2 generation, mutant C was less toxic to Vibrio alginolyticus in a plankton/ bacteria co-culture system as compared to parental C. marina. In contrast, no significant difference in H_2O_2 production between mutant C and parental C. marina was observed. These results may provide experimental evidence for a direct connection between the toxic effect of C. marina against V. alginolyticus and the production of superoxide anion. Although the detailed mechanism of the production of superoxide anion by C. marina is still unclear, our mutant strain isolated in this study may be a good tool for the clarification of the mechanism of O^-_2 generation by C. marina.

• 社団法人日本農芸化学会の論文
• 1999-11-23

著者

• Oda Tatsuya

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Oda Tatsuya

  Div. Of Biochemistry Fac. Of Fisheries Nagasaki Univ.

• Oda T

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Tatsuya Oda

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Oda T

  Nagasaki Univ. Nagasaki Jpn

• Kim D

  Jeju Center Korea Basic Science Institute (kbsi)

• ISHIMATSU ATSUSHI

  Institute for East China Sea Research, Nagasaki University

• Ishimatsu A

  Nagasaki Univ. Nagasaki

• Ishimatsu Atsushi

  Inst. For East China Sea Res. Nagasaki Univ.

• Ishimatsu Atsushi

  Marine Research Institute Nagasaki University

• Oda T

  New Industry Creation Hatchery Center Tohoku University

• Kim Daekyung

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• MURAMATSU Tsuyoshi

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Okamoto Tarou

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Kim Daekyung

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Ishimatsu Atsushi

  Noma Fisheries Station Nagasaki University

• Muramatsu Tsuyoshi

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Muramatsu Tsuyoshi

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo

• Muramatsu T

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo

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