Hemagglutinating Activity of Extracellular Alkaline Metalloendopeptidases from Vibrio sp. NUF-BPP1

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概要

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• Alkaline metalloendopeptidase (metalloprotease) AP1 (48 kDa) from Vibrio sp. isolated from the intestine of a five-barred goatfish (Parupeneus trifasciatus) was reported in our previous paper to produce AP2 (36 kDa) by releasing a peptide fragment (molecular mass of about 12 kDa) from the C-terminal end of AP1 by autodigestion.^<1)> AP1 strongly agglutinated fish (flounder, Paralichthys olivaceus) and rabbit erythrocytes, and weakly chicken erythrocytes. In contrast, AP2 had no significant hemagglutinating activity toward any erythrocytes tested, except for weak activity on flounder erythrocytes, suggesting that the C-terminal region of AP1 may be required for the strong hemagglutinating activity. The optimum temperature for the hemagglutinating activity of AP1 was found to be lower than that for the proteolytic activity. At acidic pHs (below pH 7.5), the hemagglutinating activity of AP1 decreased, and its pH profile resembled that of the proteolytic activity. The hemagglutinating activity of AP1 was not observed in the presence of o-phenanthroline or synthetic and proteinous substrates, but different kinds of saccharides and lipids had no effect. While the proteolytic activity of AP1 was not affected by CaCl_2,the hemagglutinating activity of AP1 decreased with increases in CaCl_2 concentrations. These results suggested that the hemagglutinating activity of these proteases (AP1 and AP2) was most likely caused by their proteolytic action on erythrocyte cell surfaces.

• 社団法人日本農芸化学会の論文
• 1998-06-23

著者

• Oda Tatsuya

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Oda T

  Nagasaki Univ. Nagasaki Jpn

• ISHIMATSU ATSUSHI

  Institute for East China Sea Research, Nagasaki University

• Ishimatsu A

  Nagasaki Univ. Nagasaki

• Ishimatsu Atsushi

  Inst. For East China Sea Res. Nagasaki Univ.

• Ishimatsu Atsushi

  Marine Research Institute Nagasaki University

• Oda T

  New Industry Creation Hatchery Center Tohoku University

• MURAMATSU Tsuyoshi

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Okamoto Tarou

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Fukuda Kohsai

  Department Of Agricultural Chemistry Kyushu University:(present Address) Department Of Applied Micro

• FUKUDA Kazumasa

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• HAMAGUCHI Nami

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Fukuda Kazumasa

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Ishimatsu Atsushi

  Noma Fisheries Station Nagasaki University

• Muramatsu Tsuyoshi

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Muramatsu Tsuyoshi

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo

• Muramatsu T

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo

• Fukuda K

  Unit Of Chemistry Faculty Of Engineering Kansai University

• Hamaguchi Nami

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

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