A Discrepancy in Superoxide Scavenging Activity between the ESR-Spin Trapping Method and the Luminol Chemiluminescence Method
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概要
- 論文の詳細を見る
In a previous study of ours, the superoxide scavenging activity of aqueous extracts from dinophycean red tide flagellates was detected by an electron spin resonance (ESR)-spin trapping method, but not by an L-012 (luminol analog)-dependent chemiluminescence (CL) method. To investigate the discrepancy between the two methods, the effect of ferric–protein complexes on superoxide scavenging activity was examined. The reduced signal intensity of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO)–OOH due to superoxide dismutase (SOD) did not change with the addition of horseradish peroxidase (HRP), while the reduced CL response due to SOD was restored by the addition of different concentrations of HRP. Since HRP is a ferric–protein complex, the effects of other ferric–protein complexes, catalase and hemoglobin, on the reduced CL response due to SOD were examined, and similar results were obtained. As is the case with SOD, the reduced CL response activity due to an aqueous extract from a raphidophycean red tide flagellate, Chattonella ovata, was also enhanced by HRP, catalase, and hemoglobin. ESR spectra analyzed at 77 K indicated that aqueous extracts of Gymnodinium impudicum and Alexandrium affine, both of which are dinophycean red tide flagellates, contained a ferric–protein complex, and that an extract of C. ovata did not. These results suggest that the presence of such a ferric–protein complex is a causative factor in the discrepancy between the ESR and luminol CL methods when determining superoxide scavenging activity.
- 社団法人 日本農芸化学会の論文
- 2007-06-23
著者
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ODA Tatsuya
Faculty of Fisheries, Nagasaki University
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Oda Tatsuya
Div. Of Biochemistry Fac. Of Fisheries Nagasaki Univ.
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Oda T
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Sato Emiko
New Industry Creation Hatchery Center Tohoku University
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Tatsuya Oda
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Niwano Yoshimi
New Industry Creation Hatchery Center, Tohoku University
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Kohno Masahiro
New Industry Creation Hatchery Center, Tohoku University
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Oda T
Nagasaki Univ. Nagasaki Jpn
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Kim D
Jeju Center Korea Basic Science Institute (kbsi)
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Oda T
New Industry Creation Hatchery Center Tohoku University
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MATSUYAMA Yukihiko
Seikai National Fisheries Research Institute
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Oda Tatsuya
Faculty Of Fisheries Nagasaki University
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Okamoto Tarou
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Niwano Yoshimi
New Ind. Creation Hatchery Center Tohoku Univ.
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Kohno Masahiro
New Ind. Creation Hatchery Center Tohoku Univ.
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Kim Daekyung
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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MOKUDAI Takayuki
New Industry Creation Hatchery Center, Tohoku University
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Matsuyama Yukihiko
Fisheries Research Agency, National Research Institute of Fisheries and Environment of Inland Sea
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KIM Daekyung
Research Center for Functional Food Materials, Sunny Health Co., Ltd.
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Oda T
Faculty Of Fisheries Nagasaki University
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Matsuyama Y
Seikai National Fisheries Research Institute
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Matsuyama Yukihiko
Fisheries Res. Agency Hiroshima Jpn
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Kamibayashi Masato
Faculty Of Fisheries Hokkaido University
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Mokudai Takayuki
New Industry Creation Hatchery Center Tohoku University
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Niwano Yoshimi
Tohoku University Graduate School Of Dentistry Laboratory For Redox Regulation
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Niwano Yoshimi
Tohoku University Graduate School Of Dentistry
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