Non-reductive Scavenging of 1,1-Diphenyl-2-picrylhydrazyl (DPPH) by Peroxyradical : A Useful Method for Quantitative Analysis of Peroxyradical
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概要
- 論文の詳細を見る
A stable radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) has long been used as a convenient method for the antioxidant assay of biological materials such as cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds (hydroquinone, pyrogallol, etc). In this study, non-reductive scavenging of DPPH was investigated by electron spin resonance (ESR) analyses for the purpose of developing a useful method for quantitative determination of peroxyradical. Since DPPH was degraded in the presence of peroxyradical derived from UV-irradiated benzoylperoxide and the peroxyradical-induced degradation of DPPH was inhibited by the addition of a spin trapping agent 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), it is concluded that DPPH is non-reductively scavenged by peroxyradical. Therefore, it is suggested that DPPH could be a useful agent for the quantitative measurement of peroxyradical.
- 公益社団法人日本薬学会の論文
- 2005-06-01
著者
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Niwano Yoshimi
New Industry Creation Hatchery Center, Tohoku University
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Kohno Masahiro
New Industry Creation Hatchery Center, Tohoku University
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Kitagawa Akio
Business Development Division Sunny Health Co. Ltd.
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Niwano Yoshimi
New Ind. Creation Hatchery Center Tohoku Univ.
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Kohno Masahiro
New Ind. Creation Hatchery Center Tohoku Univ.
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NISHIZAWA Masahiro
New Industry Creation Hatchery Center, Tohoku University
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NISHIMURA Minemitsu
Business Development Division, Sunny Health Co., Ltd.
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Nishimura Minemitsu
Business Development Division Sunny Health Co. Ltd.
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Nishizawa Masahiro
New Industry Creation Hatchery Center Tohoku University
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Nishizawa Masahiro
New Ind. Creation Hatchery Center Tohoku Univ.
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