Generation of reactive oxygen species from conventional laboratory media(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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We previously reported that superoxide anion was generated from glucose-peptone-meat extract medium. We found that superoxide anion was generated from other conventional laboratory media, Nutrient Broth, Tryptic Soy Broth, and Mueller Hinton Broth as well as glucose-peptone-meat extract medium. The glucose-peptone-meat extract medium consisting of 1% glucose, 0.5% peptone, 0.5% meat extract, and 0.3% NaCl showed the highest superoxide anion generation among the media tested. The meat extract and peptone, which are natural nutrient sources in the medium, showed an increase in absorbance intensity by WST-1 formazan products upon reaction with superoxide anion. The specific type of reactive oxygen species was measured by SOD-inhibitable reduction of cytochrome c and WST-1 (for superoxide anion), Fluoro H_2O_2^<TM> kit (for hydrogen peroxide), hydroxyphenyl fluorescein (for hydroxyl radical), and Singlet Oxygen Sensor Green (for singlet oxygen). The generation of hydroxyl radical was also evaluated by ESR-spin trapping method. We detected superoxide anion, hydrogen peroxide, and hydroxyl radical but not singlet oxygen. These results suggest that the superoxide anion spontaneously generated from glucose-peptone-meat extract medium is finally converted to hydroxyl radical.
著者
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Nakashima Takuji
Kitasato Institute for Life Sciences, Kitasato University
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Nakamura T
Kitasato Institute For Life Sciences Kitasato University
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Sato Emiko
New Industry Creation Hatchery Center Tohoku University
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OMURA Satoshi
Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato Uni
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Seki Tamae
Kitasato Institute for Life Sciences, Kitasato University
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Matsumoto Atsuko
Kitasato Institute for Life Sciences, Kitasato University
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Miura Hiromi
Kitasato Institute for Life Sciences, Kitasato University
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Niwano Yoshimi
New Industry Creation Hatchery Center, Tohoku University
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Kohno Masahiro
New Industry Creation Hatchery Center, Tohoku University
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Takahashi Yoko
Kitasato Institute for Life Sciences, Kitasato University
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Omura Satoshi
Kitasato Institute For Life Sciences Kitasato University
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Seki Tamae
Kitasato Institute For Life Sciences Kitasato University
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Morimoto Hiromi
School Of Pharmaceutical Sciences Kitasato University
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Miura Hiromi
Kitasato Institute For Life Sciences Kitasato University
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Takada Y
Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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Niwano Yoshimi
New Ind. Creation Hatchery Center Tohoku Univ.
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Kohno Masahiro
New Ind. Creation Hatchery Center Tohoku Univ.
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Takahashi Yoko
Kitasato Institute For Life Sciences Kitasato University
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Nagai T
The Kitasato Institute
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Matsumoto Atsuko
Kitasato Institute For Life Sciences Kitasato University
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Omura S
Kitasato Institute For Life Sciences Kitasato University
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NAKAMURA Tomonori
The Kitasato Institute
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Tanaka Y
Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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Nakashima Takuji
Division Of Biochemistry Faculty Of Fisheries Nagasaki University:nagasaki Prefecture Collaboration
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Omura Satoshi
Kitasato Institute For Life Sciences And Graduate School Of Infection Control Sciences Kitasato Univ
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Nakashima Takuji
Kitasato Institute For Life Sciences Kitasato University
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Nishihara Aki
Research Center For Tropical Disease The Kitasato Institute
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Kamibayashi Masato
Faculty Of Fisheries Hokkaido University
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Taniguchi Y
Kitasato Institute For Life Sciences Kitasato University
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Tanaka Y
Research Center For Biological Function
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Tanaka Y
Research Center For Biological Function The Kitasato Institute
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Nagai Takayuki
Oriental Medicine Res. Center Of The Kitasato Inst. Tokyo Jpn
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Matsumoto A
Research Center For Biological Function The Kitasato Institute
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Takahashi Yoko
Graduate School Of Agricultural Science Tohoku University
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Omura Satoshi
School Of Pharmaceutical Sciences Kitasato University:research Center For Biological Function The Ki
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Niwano Yoshimi
Tohoku University Graduate School Of Dentistry Laboratory For Redox Regulation
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Takahashi Yoko
The Kitasato Institute
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Niwano Yoshimi
Tohoku University Graduate School Of Dentistry
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Nishihara Tsukashima
Research Center For Tropical Diseases Kitasato Institute For Life Sciences Kitasato University
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Matsumoto Atsuko
Kitasato Institute For Life Sciences And Graduate School Of Infection Control Sciences Kitasato University
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Matsumoto Atsuko
Kitasato Institute
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Takahashi Yoko
Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University
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Matsumoto Atsuko
Kitasato Instit. Life Sci., Kitasato Univ.
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Takahashi Yoko
Kitasato Instit. Life Sci., Kitasato Univ.:Grad. Sch. Infect. Con. Sci., Kitasato Univ.
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Omura Satoshi
Kitasato Institute
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NAKASHIMA Takuji
Kitasato Research Organization for Infection Control Sciences, Kitasato University
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