Role of Dexamethasone and Oncostatin M on the Formation of Vacuoles in Human Fetal Liver Cells(Molecular and Cell Biology)
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概要
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Combined treatment with dexamethasone and oncostatin M (DEX/OSM) or interleukin-6 (DEX/IL-6) resulted in the appearance of numerous large vacuoles in human fetal liver (HFL) cells and showed synergistic effects on the formation of vacuoles. The number of vacuoles formed by DEX, DEX/OSM, or DEX/IL-6 was significantly suppressed by RU-486, a glucocorticoid receptor antagonist. On the other hand, the size of vacuoles formed by OSM, IL-6, DEX/OSM, or DEX/IL-6 was significantly decreased to about 65% by madindoline A (MDL-A), which is a non-peptide antagonist of gp130 and an inhibitor of cytokines, such as IL-6, mediated by gp130 homodimerization, while RU-486 did not affect the size of vacuoles. Expression of IL-6 mRNA in HFL cells was markedly induced by OSM. Expression of IL-6R mRNA was induced by DEX. These results indicate that DEX contributes to the formation of vacuoles through glucocorticoid receptors and that OSM and IL-6 contribute to enlargement of these vacuoles.
- 2009-02-01
著者
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SUNAZUKA Toshiaki
Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato Uni
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OMURA Satoshi
Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato Uni
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Omura Satoshi
Kitasato Institute For Life Sciences Kitasato University
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TERAMOTO Tsuyoshi
Department of Pharmacy, Shinshu University Hospital
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MATSUNAGA Tamihide
Department of Pharmacy, Shinshu University Hospital
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TOBA Mie
Department of Pharmacy, Shinshu University Hospital
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OHMORI Shigeru
Department of Pharmacy, Shinshu University Hospital
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Sunazuka Toshiaki
Kitasato Institute For Life Sciences Kitasato University
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Omura Satoshi
Kitasato Institute For Life Sciences And Graduate School Of Infection Control Sciences Kitasato Univ
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Toba Mie
Department Of Pharmacy Shinshu University Hospital
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Ohmori Shigeru
Department Of Pharmacy Shinshu University Hospital
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Matsunaga Tamihide
Department Of Pharmacy Shinshu University Hospital
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Teramoto Tsuyoshi
Department Of Pharmacy Shinshu University Hospital
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Sunazuka Toshiaki
Kitasato Institute for Life Sciences and Graduate School of Infection Control Science, Kitasato University
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OHMORI Shigeru
Department of Biochemical Pharmacology and Biotoxicology, Faculty of Pharmaceutical Sciences, Chiba University
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Matsunaga Tamihide
Department of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University
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Omura Satoshi
Kitasato Institute
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