A Carbonyl Reductase-Catalyzing Reduction of N^3-Phenacyluridine in Rabbit Liver

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概要

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• Carbonyl reductase activity for a novel hypnotic, N^3-phenacyluridine, was mainly localized in the cytosol fraction of rabbit liver. The enzyme (N^3-phenacyluridine reductase) which catalyzes the reduction of N^3-phenacyluridine to N^3-α-hydroxy-β-phenethyluridine was surified from the cytosolic fraction of rabbit liver by various chromatographic techniques (DEAE-Sephacel, Red Sepharose CL-6B and hydroxylapatite). N^3-Phenacyluridine reductase had a minimum molecular weight of 39 kDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis. This enzyme required reduced nicotinamide adenine dinucleotide phosphate (NADPH) as a cofactor and its optimal pH was 7.5. Flavonoids (quercetin and quercitrin) were potent inhibitors of the enzyme, but pyrazole or harbital had little effect. The apparent K_m and V_<max> values of the enzyme for the reduction of N^3-phenacyluridine were 0.32 mM and 8.7 units/mg protein, respectively. A variety of carbonyl compounds, including N^3-phenacyluridine, were effectively reduced by the enzyme. However, the enzyme purified from rabbit liver differs in several respects from known carbonyl reductases in rabbit liver.

• 社団法人日本薬学会の論文
• 2000-08-01

著者

• YAMAMOTO IKUO

  Department of Laboratory Medicine, Miyazaki Medical College

• 景山 節

  京大・霊長類研・生化学

• Yamamoto Ikuo

  Department Of Hygienic Chemistry School Of Pharmaceutical Sciences Kyushu University Of Health And W

• Kimura Toshiyuki

  Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

• Watanabe Kazuhito

  Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

• Kondo Shigemi

  Nissui Pharmaceutical Co. Ltd.

• MATSUNAGA Tamihide

  Department of Pharmacy, Shinshu University Hospital

• YAMAMOTO Ikuo

  School of Pharmaceutical Sciences, Kyushu University of Health and Welfare

• KOBANA Kiyofumi

  Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

• Yamamoto Ikuo

  北陸大学 薬 衛化

• Yamamoto Ikuo

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University

• YAMAMOTO IKUO

  Hygienic Chemistry

• Kimura T

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University

• 景山 節

  京都大学霊長類研究所人類進化モデル研究センター

• 景山 節

  京大・霊長研

• Kimura Toshiyuki

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University

• Watanabe Kazuhito

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University

• Watanabe Kazuhito

  Department Of Chemistry And Chemical Engineering Faculty Of Engineering Yamagata University

• Kimura T

  Department Of Medicinal Chemistry Center For Frontier Research In Medicinal Science 21st Century Coe

• Kondo S

  Kyoritsu Coll. Pharmacy Tokyo Jpn

• Yamamoto I

  Hokuriku Univ. Kanazawa Jpn

• Kondo Shin-ichi

  Dep. Of Pharmaceutical Physical Chemistry Gifu Pharmaceutical Univ.

• Yamamoto I

  Hygienic Chemistry

• Matsunaga Tamihide

  Department Of Pharmacy Shinshu University Hospital

• Watanabe Kazuhito

  Faculty Of Pharmaceutical Sciences Kyushu University

• Yamamoto Ikuo

  Hygienic Chemistry School Of Pharmacy Hokuriku University

• Kobana Kiyofumi

  Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University

• Yamamoto Ikuo

  Department Of Bioapplied Chemistry Faculty Of Engineering Osaka City University

• Kimura T

  Peptide Inst. Inc. Minoh Jpn

• Matsunaga Tamihide

  Department of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University

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