Fluorometric Determination of Hydrogen Peroxide Based on Formation of Amprochrome

概要

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A fluorometric determination of H_2O_2 based on the formation of amprochrome was developed. Amprochrome is an oxidation product of amprolium, an agent used for treating coccidiosis of chickens, and has a strong fluorescence. Though potassium ferricyanide has been used for oxidizing amprolium, H_2O_2 is also considered to be useful for the reaction. The analytical procedure was as follows. To a sample solution containing a suitable quantity of H_2O_2,1 ml of amprolium (500 μg/ml), NaOH (10%) and peroxidase solution (20 units/ml) were added. The mixture was shaken for 45 min at room temperature and amprochrome produced was extracted with 10 ml of isoBuOH after saturating the solution with sodium sulfate. Relative fluorescence intensity of the separated isoBuOH layer was measured (excitation wavelength 400 nm and emission wavelength 460 nm) against that of 10^<-6>M quinine sulfate in 0.1 N H_2SO_4. The calibration plot was linear up to H_2O_2 0.2 μg/tube. On the basis of the proposed method, remaining H_2O_2 in Japanese noodles (udon) could be determined with a recovery of 82.4-108%.
公益社団法人日本薬学会の論文
1986-06-30