THE PROPERTIES OF UDP-GLUCURONYLTRANSFERASE FOR CANNABINOIDS IN RAT LIVER MICROSOMES
スポンサーリンク
概要
- 論文の詳細を見る
The glucuronidation of Δ^9-tetrahydrocannabinol (Δ^9-THC), cannabidiol (CBD) and cannabinol (CBN) in rat liver microsomes was studied. The enzyme activities for the cannabinoids were 26.3 (Δ^9-THC), 52.9 (CBD) and 104.8 (CBN) pmol/min/mg protein. The apparent K_m values of UDP-glucuronyltransferase for the cannabinoids were 0.29 (Δ^9-THC), 0.18 (CBD) and 2.78 (CBN) mM, while V_<max> were 40.3 (Δ^9-THC), 104.9 (CBD) and 593.3 (CBN) pmol/min/mg protein. Following treatment of rats with 3-methylcholanthrene, the enzyme activities for Δ^9-THC, CBD and CBN were increased 132,43 and 1198%, respectively, whereas the corresponding increases in microsomes from phenobarbital-treated rats were 127,13 and 97%, respectively. The cannabinoid glucuronidation was activated 2 to 3 folds by the addition of UDP-N-acetylglucosamine, but not activated by the addition of Triton X-100 in vitro. The properties of cannabinoid UDP-glucuronyltransferase were discussed from the above results.
- 社団法人日本薬学会の論文
著者
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YOSHIMURA HIDETOSHI
Faculty of Pharmaceutical Sciences, Kyushu University
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OGURI Kazuta
School of Pharmaceutical Sciences, Kyushu University of Health and Welfare
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YAMAMOTO Ikuo
School of Pharmaceutical Sciences, Kyushu University of Health and Welfare
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Yamamoto Ikuo
北陸大学 薬 衛化
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WATANABE KAZUHITO
School of Pharmacy, Hokuriku University
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KITA MICHIYO
School of Pharmacy, Hokuriku University
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Yoshimura Hidetoshi
Faculty Of Pharmaceutical Sciences Kyushu University
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Oguri Kazuta
School Of Pharmaceutical Sciences Kyushu University Of Health And Welfare
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Oguri Kazuta
School Of Pharmacy Hokuriku University
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Kita Michiyo
School Of Pharmacy Hokuriku University
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Watanabe Kazuhito
Faculty Of Pharmaceutical Sciences Kyushu University
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Yamamoto Ikuo
School Of Pharmaceutical Sciences Kyushu University Of Health And Welfare
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