Novel Extracellular Alkaline Metalloendopeptidases from Vibrio sp. NUF-BPP1 : Purification and Characterization
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概要
- 論文の詳細を見る
We found two types of novel alkaline metalloendopeptidases (AP1 and AP2) from a marine bacterium, isolated from the intestine of a five-barred goatfish (Parupeneus trifasciatus) and identified as Vibrio sp. (NUF-BPP1). We studied the structure-function relationship of these marine bacterial proteases. The electrophoretically homogeneous proteases had a molecular mass of 48 kDa for AP1 and 36 kDa for AP2 on SDS-PAGE, and showed optimum activity at around pH 9.5-10.0 (casein as substrate). Calcium chloride (5 mM) stabilized the activities over pH 6-11, but o-phenanthroline and EDTA inhibited the activities of both AP1 and AP2. The EDTA-inactivated proteases were partly restored to activity by addition of either zinc or calcium. Sodium chloride (3.5M) increased the activities toward Z-Gly-Leu-NH_2. N-Terminal sites of hydrophobic amino acid residues (Leu, Ile, Phe, Tyr, and Trp) of the peptide substrates were cleaved by AP1 and by AP2. Autolysis of AP1 in the absence of calcium ion probably produced AP2 by releasing a fragment (molecular mass of about 12kDa) from the C-terminal end of AP1.
- 社団法人日本農芸化学会の論文
- 1997-01-23
著者
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Oda T
Nagasaki Univ. Nagasaki Jpn
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Fukuda Kohsai
Department Of Agricultural Chemistry Kyushu University:(present Address) Department Of Applied Micro
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FUKUDA Kazumasa
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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Fukuda Kazumasa
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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Muramatsu Tsuyoshi
Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo
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Muramatsu T
Division Of Biochemistry Faculty Of Fisheries Nagasaki University:(present Address)department Of Foo
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Fukuda K
Unit Of Chemistry Faculty Of Engineering Kansai University
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Hasuda Katsumi
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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Oda Tatsuya
POLA Pharmaceutical R & D Laboratory
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Yoshimura Hiroshi
Division of Biochemistry, Faculty of Fisheries, Nagasaki University
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Muramatsu Tsuyoshi
Kakuyo-Maru, Faculty of Fisheries, Nagasaki University
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Hasuda K
Division Of Biochemistry Faculty Of Fisheries Nagasaki University
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