Specific Inhibition by Cyclodextrins of Raw Starch Digestion by Fungal Glucoamylase

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概要

• 論文の詳細を見る

• α-, β-, and γ-cyclodextrins (CDs) completely inhibited raw starch digestion by glucoamylase I (GA I, MW 90,000) from Aspergillus awamori var. kawachi, and inhibited by 85% the raw starch adsorption of GA I at the CD concentrations of 1-5 ImM. CDs at 1-5 mM did not inhibit gelatinized starch hydrolysis by GA I, but at the concentration of 50 mM, they inhibited such hydrolysis slightly. GA I was specifically adsorbed onto CD-Sepharose 6B, but glucoamylase I' (GA I', MW 73,000), which does not adsorb onto or digest raw starch, from the same strain was not adsorbed onto that gel. The adsorption of the glucoamylases onto raw starch and CD-Sepharose 6B was correlated to their digestion of raw starch. The hydrophobic adsorption of GA I onto CDs and raw starch occurred competitively at the Cp region, which is on the C-terminal side of Gp-I in the site for raw starch affinity of GA I, and inclusion complexes were formed.

• 社団法人日本農芸化学会の論文
• 1992-04-23

著者

• Hayashida Shinsaku

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University

• SAKAMOTO Junichi

  Department of Young Leaders' Program in Health Care Administration, Nagoya University Graduate Schoo

• Goto M

  Univ. Tokyo Tokyo Jpn

• Hayashida Shinsaku

  Department Of Agricultural Chemistry Kyushu University:corresponding Author

• Hayashida Shinsaku

  Department Of Agricultural Chemistry

• FUKUDA Kohsai

  Department of Agricultural Chemistry, Kyushu University

• TERAMOTO Yuji

  Department of Agricultural Chemistry, Kyushu University

• GOTO Masatoshi

  Department of Agricultural Chemistry, Kyushu University

• MITSUIKI Shinji

  Department of Agricultural Chemistry, Kyushu University

• Teramoto Y

  Kyushu Univ. Fukuoka Jpn

• Teramoto Yuji

  Department Of Agricultural Chemistry Kyushu University

• Fukuda Kohsai

  Department Of Agricultural Chemistry Kyushu University:(present Address) Department Of Applied Micro

• Fukuda Kohsai

  Department Of Agricultural Chemistry

• Goto Masatoshi

  Department Of Agricultural Chemistry Kyushu University

• Sakamoto Junichi

  Department Of Epidemiological And Clinical Research Information Management Kyoto University Graduate

• Teramoto Y

  Department Of Agricultural Chemistry Kyushu University:(present Address) Department Of Applied Micro

• Fukuda Kazumasa

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Mitsuiki S

  Kyushu Sangyo Univ. Fukuoka Jpn

• Mitsuiki Shinji

  Department Of Applied Chemistry And Biochemistry Kyushu Sangyo University

• Mitsuiki Shinji

  Department Of Agricultural Chemistry Kyushu University

• Fukuda K

  Unit Of Chemistry Faculty Of Engineering Kansai University

• Teramoto Y

  Department Of Agricultural Chemistry Kyushu University

• Hayashida S

  Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University

• Sakamoto Junichi

  Department Of Agricultural Chemistry Kyushu University

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