A Novel Method for Breeding Polyploid Cells by Heat-induced Endomitotic Diploidization in Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
Endomitotic diploidization occurred as a result of heat treatment of spores during germination of the industrial yeast S. cerevisiae. Polyploid cells were obtained in both heterothallic and homothallic strains. In the heterothallic strains, the resultant diploid cells, with the ability to mate, were used to construct tetraploids. In the homothallic strains, tetraploid cells were obtained directly after a mating-type switch. In each strain, the highest frequency (approximately 10% of viable cells) of diploidization was obtained when spores were cultured for 30 min in liquid medium to initiate germination followed by subsequent heat treatment at, 55℃ for 10 min.
- 社団法人日本農芸化学会の論文
- 1993-12-23
著者
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Hayashida Shinsaku
Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University
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Hayashida Shinsaku
Department Of Agricultural Chemistry Kyushu University:corresponding Author
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Hayashida Shinsaku
Department Of Agricultural Chemistry
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Tani Yoshinobu
Fukutokucho Co., Ltd.
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Kurokui Takamasa
Department of Agricultural Chemistry, Kyushu University
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Tomohiro Yukio
Fukutokucho Co., Ltd.
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Miyata Akira
Fukutokucho Co., Ltd.
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Tani Yoshinobu
Fukutokucho Co. Ltd.
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Tomohiro Yukio
Fukutokucho Co. Ltd.
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Kurokui Takamasa
Department Of Agricultural Chemistry Kyushu University
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Hayashida S
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
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Miyata A
Niaes
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