Characteristics of Raw-Starch-Digesting Glucoamylase from Thermophilic Rhizomucor pusillus
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概要
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A newly isolated thermophilic fungus, NH-139,identified as Rhizomucor pusillus (Lindt) Schipper produced only a single form of raw-starch-adsorbable, raw-starch-digesting glucoamylase on solid wheat bran medium at 45℃. The electrophoretically homogeneous preparation of glucoamylase, molecular weight 68,000,had its optimal temperature on gelatinized starch at 65℃ and on raw corn starch at 50℃. However, this raw-starch-digesting glucoamylase, unlike other glucoamylases, could not completely hydrolyze glycogen but hydrolyzed it to the extent of 80% as glucose, and is classified as type B. The subtilisin-modified glucoamylase of this strain, molecular weight 60,000,still belonged to typ B in the hydrolysis curve on glycogen and lost the ability to digest and adsorb onto raw starch.
- 社団法人日本生物工学会の論文
- 1987-08-25
著者
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Hayashida Shinsaku
Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University
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Hayashida Shinsaku
Department Of Agricultural Chemistry Kyushu University
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Hayashida Shinsaku
Department Of Agricultural Chemistry
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Kanlayakrit Werasit
Department Of Agricultural Chemistry Kyushu University
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Nakao Masahiro
Suntory Co.
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ISHIMATSU KEISUKE
Sumitomo Chemical Co.,
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Ishimatsu Keisuke
Sumitomo Chemical Co.
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