Molecular Cloning of the Glucoamylase I Gene of Aspergillus awamori var. kawachi for Localization of the Raw-starch-affinity Site(Microbiology & Fermentation Industry)

スポンサーリンク

概要

• 論文の詳細を見る

• The raw-starch-digesting glucoamylase I (GAI) of Aspergillus awamori var. kawachi contains a glycopeptide I (Gp-I) region as the raw-starch-affinity site essential for its raw-starch-adsorption and raw-starch-digestion. Molecular cloning of the GAI gene and analysis of its nucleotide sequence revealed some nucleotide replacements, in comparison with the Aspergillus niger and Aspergillus awamori glucoamylase genes. The deduced total amino acid sequence of GAI contained 35 replacements and one deletion, of amino acid residues, and therefore a total of 615 residues, in comparison with the A. niger and A. awamori glucoamylases. The raw-starch-adsorbable Gp-I region in GAI was located between Ala^<470> and Val^<514> near the C-terminal, consisting of ATGGTTTTATTTGSGGVTSTSKTTTTASKTSTTTSSTSCTTPTAV.

• 社団法人日本農芸化学会の論文
• 1989-04-23

著者

• Kuhara Satoru

  Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

• Ohta Kazuyoshi

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Hayashida Shinsaku

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University

• Sakaki Y

  Riken Genomic Sciences Center

• Sakaki Y

  Genomic Sciences Center Riken Yokohama Institute

• Hayashida Shinsaku

  Department Of Agricultural Chemistry Kyushu University

• Hayashida Shinsaku

  Department Of Agricultural Chemistry

• Hayashida Shinsaku

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University

• FUKUDA Kohsai

  Department of Agricultural Chemistry, Kyushu University

• KURODA Kazutaka

  Department of Agricultural Chemistry

• SAKAI Yoshiyuki

  Research Laboratory for Genetic Information, Kyushu University

• Fukuda Kohsai

  Department Of Agricultural Chemistry

• Kuhara Satoru

  Department Of Agricultural Chemistry

• Sakaki Yoshiyuki

  Research Laboratory For Genetic Information Kyushu University

• Ohta Kazuyoshi

  Department Of Agricultural Chemistry

• Sakaki Yoshiyuki

  Human Genome Center Institute Of Medical Science University Of Tokyo

• Sakai Yoshiyuki

  Research Institute For A Tomic Energy Osaka City University

• Sakaki Y

  Univ. Tokyo Tokyo

• Hayashida S

  Department Of Agricultural Chemistry Kyushu University

• Ohta K

  Univ. Osaka Prefecture Osaka Jpn

関連論文

• Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• An extracellular endo-1,4-β-xylanase from Aspergillus japonicus : Purification, properties, and characterization of the encoding gene(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Cell-associated β-xylosidase from Aureobasidium pullulans ATCC 20524 : Purification, properties, and characterization of the encoding gene(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• An Absorption Mechanism for the Decolorization of Melanoidin by Rhizoctonia sp. D-90
• Appearance of Osteonectin-expressing Fibroblastic Cells in Early Rat Stomach Carcinogenesis and Stomach Tumors Induced with N-Methyl-N'-nitro-N-nitrosoguanidine
• Rare Occurrence of ras and p53 Gene Mutations in Mouse Stomach Tumors Induced by N-Methyl-N-nitrosourea
• Specific Inhibition by Cyclodextrins of Raw Starch Digestion by Fungal Glucoamylase
• A Novel Raw-Starch-Digesting Yeast α-Amylase from Lipomyces starkeyi HN-606
• The Hyperdigestion of Raw Starch by a Carbohydrate-Rich Glucoamylase from a Protease- and Glycosidase-Negative Mutant of Aspergillus awamori var. kawachi F-2035
• Molecular Cloning of the Glucoamylase I Gene of Aspergillus awamori var. kawachi for Localization of the Raw-starch-affinity Site(Microbiology & Fermentation Industry)
• Evidence for Post-translational Generation of Multiple Forms of Aspergillus awamori var. kawachi Glucoamylase(Biological Chemistry)
• A Novel Type of Life Cycle "Delayed Homothallism" in Saccharomyces cerevisiae wy2 Showed Slow Interconversion of Mating-type
• A Novel Method for Breeding Polyploid Cells by Heat-induced Endomitotic Diploidization in Saccharomyces cerevisiae
• Frequent Occurrence of Diploid a or α Sporogenous Mater Cells and Construction of Ploidy Series in the Sake Yeast Kyokai No. 9
• Growth Promotion of Fluorescent Pseudomonads and Control of Potato Common Scab in Field Soil with Non-antibiotic Actinomycete-biofertilizer
• Isolation of Anti-Algal Pseudomonas stutzeri Strains and Their Lethal Activity for Chattonella antiqua(Microbiology & Fermentation Industry)
• Control of Potato Common Scab with an Antibiotic Biofertilizer Produced from Swine Feces Containing Streptomyces albidoflavm CH-33
• Lipid Composition of a Thermotolerant Yeast, Hansenula polymorpha
• Antitumor Activity of an Alkali Extract of Clostridium saccharoperbutylacetonicum Cells
• Evidence for Raw Starch-affinity Site on Aspergillus awamori Glucoamylase I
• Production and Purification of a Novel Type of CMCase from Humicola grisea var. thermoidea YH-78
• Raw Starch-digestive Chitin-immobilized Amylase from a Protease-Glycosidase-less Mutant of Aspergillus awamori var. kawachi
• Enzymatic Decolorization of Melanoidin by Coriolus sp. No. 20
• High Concentration-Ethanol Fermentation of Raw Ground Corn
• Purification and Properties of Thermostable Xylanase from Talaromyces byssochlamydoides YH-50
• Raw Starch-digestive Glucoamylase Productivity of Protease-less Mutant from Aspergillus awamori var. kawachi
• Promotive and Inhibitory Effects of Raw Starch Adsorbable Fragments from Pancreatic α-Amylase on Enzymatic Digestions of Raw Starch(Biological Chemistry)
• Multiplicity and Preferential Inactivation by Proteolysis as to Raw Starch-digestibility of Bacterial α-Amylases(Microbiology & Fermentation Industry)
• Reproductive parameters of the chub mackerel Scomber japonicus estimated from human chorionic gonadotropin-induced final oocyte maturation and ovulation in captivity
• Production of Potato Common Scab-antagonistic Biofertilizer from Swine Feces with Streptomyces albidoflavus(Microbiology & Fermentation Industry)
• Conversion of Geotrichum candidum Endocellulase I to Endocellulase II by Limited Proteolysis(Biological Chemistry)
• Production and Characteristics of Two Types of Endocellulase from Geotrichum candidum(Biological Chemistry)
• Temperature-Sensitive Mutants of a Thermotolerant Yeast, Hansenula polymorpha
• Isolation and Growth Characteristics of a Nystatin-Resistant Mutant of a Thermotolerant Yeast, Hansenula polymorpha
• 315 Lipid Composition of a Thermotolerant Yeast Hansenula polymorpha
• Structure of a New Rhamnose Polysaccharide, Clostrhamnan, Isolated from Antitumor Hot Water Extract of Clostridium saccharoperbutylacetonicum Cells(Biological Chemistry)
• Isolation and Antitumor Activity of a New Rhamnose Polysaccharide, Clostrhamman from Clostridium saccharoperbutylacetonicum
• Functional Characterization and Evolutionary Implication of the Internal 157-Amino-Acid Sequence of an Exoinulinase from Penicillium sp. Strain TN-88(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Occurrence of Two Forms of Extracellular Endoinulinase from Aspergillus niger Mutant 817
• Quantitative Expression Analysis of Inulinase Gene Cluster of Penicillium sp. Strain TN-88(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Acidophilic Xylanase from Aureobasidium pullulans : Efficient Expression and Secretion in Pichia pastoris and Mutational Analysis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Molecular Cloning and Characterization of an Exoinulinase Gene from Aspergillus niger Strain 12 and Its Expression in Pichia pastoris
• Purification and Properties of an Extracellular Exoinulinase from Penicillium sp. Strain TN-88 and Sequence Analysis of the Encoding Gene(Biochemistry & Molecular Biology)
• Purification and Characterization of an Acidophilic Xylanase from Aureobasidium pullulans var.melanigenum and Sequence Analysis of the Encoding Gene
• Inulo-Oligosaccharides : Continuous Production from Inulin by Immobilized Inulinase from Aspergillus niger and in vitro Utilization by Bifidobacteria
• Molecular Cloning and Sequence Analysis of an Endoinulinase Gene from Penicillium sp. Strain TN-88
• Transcriptional Analysis of Two Endoinulinase Genes inuA and inuB in Aspergillus niger and Nucleotide Sequences of Their Promoter Regions
• Molecular Cloning and Sequence Analysis of Two Endoinulinase Genes from Aspergillus niger
• Production, Purification and Properties of an Endoinilinase of Penicillium sp. TN-88 That Liberates Inulotriose
• Ethanol Production from Jerusalem Artichoke Tubers by Aspergillus niger and Saccharomyces cerevisiae
• Continuous Production of Fructose Syrups from Inulin by Immobilized Inulinase from Aspergillus niger Mutant 817
• Purification and Properties of an Extracellular β-Xylosidase from Aspergillus japonicus and Sequence Analysis of the Encoding Gene
• Purification and Properties of Family-10 Endo-1,4-β-Xylanase from Penicillium citrinum and Structural Organization of Encoding Gene(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Purification and Properties of an Extracellular Endo-1, 4-β-Xylanase from Penicillium citrinum and Characterization of the Encoding Gene(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Purification and Properties of an Extracellular Inulinase from Rhizopus sp. Strain TN-96(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• E2 Heavy Ion Beam Probe for Potential Measurement
• A Proposal of a New Technique of Atomic Beam-Doppler Speetroscopy for Electric Field Measurement in Plasma
• BAC library construction and BAC end sequencing of five Drosophila species : the comparative map with the D. melanogaster genome
• Familial 14-Mb deletion at 21q11.2-q21.3 and variable phenotypic expression
• The genomic structure and expression of MJD, the Machado-Joseph disease gene
• Detailed sequence analysis of the distal Down syndrome critical region and the SOD/AML region on chromosome 21
• Molecular Characterization of the Mouse mtprd Gene, a Homologue of Human TPRD: Unique Gene Expression Suggesting Its Critical Role in the Pathophysiology of Down Syndrome^1
• Identification and Cloning of a Novel cDNA Belonging to Tetratricopeptide Repeat Gene Family from Down Syndrome-Critical Region 21q22.2
• An Early Warning System for Illegal Access Based on Distributed Network Monitoring, Vol.J86-B,No.3, pp.410-418
• Mobility Management for IP/LEO Satellite Networks Using Geographical Location
• A New Scheme of Combining Advanced Packet Discard and Dynamic Bandwidth Allocation for Low Delay/Low litter Realtime Communication Using CBQ/ALTQ(Special Issue on Network Software)
• Proposal of Dynamic Bandwidth Allocation Technique for Low Delay/Low Jitter Realtime Communication and Its Evaluation by Using CBQ (Special Issue on New Developments on QoS Technologies for Information Networks)
• A Real-Time Intrusion Detection System (IDS) for Large Scale Networks and Its Evaluations (Special Issue on New Paradigms in Network Management)
• A Proposal of Event Correlation for Distributed Network Fault Management and Its Evaluation (Special Issue on Distributed Processing for Controlling Telecommunications Systems)
• The Dynamic Symptom Isolation Algorithm for Network Fault Management and Its Evaluation (Special Issue on the Latest Development of Telecommunication Research)
• Construction and Utilization Experiment of Multimedia Education System Using Satellite ETS-V and Internet (Special Issue on Educational System using Multimedia and Communication Technology)
• Problems in Management Information Retrieval for High-Speed Networks and a Proposed Solution (Special Issue on Multimedia on Demand)
• MOLECULAR CLONING OF ESTROGEN AND ANDROGEN RECEPTORS AND THEIR EXPRESSION PROFILE DURING SEX CHANGE IN THE PROTOGYNOUS WRASSE, PSEUDOLABRUS SIEBOLDI
• Steroidogenesis in Ovarian Follicles of Chub Mackerel, Scomber japonicus(Reproductive Biology)
• MOLECULAR CLONING AND CHARACTERIZATION OF DMRT GENES FROM THE PUFFERFISH TAKIFUGU RUBRIPES(Cell Biology and Morphology & Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• STEROIDOGENIC PATHWAYS IN THE VITELLOGENIC OVARIAN FOLLICLES OF THE CHUB MACKEREL, SCOMBER JAPONICUS, AND KYUSEN WRASSE, HALICHOERES POECILOPTERUS : DIFFERENT MODES OF ESTROGEN SYNTHESIS IN GONOCHORISTIC AND PROTOGYNOUS TELEOSTS
• DIURNAL RHYTHM OF GAMETOGENESIS AND SERUM SEX STEROIDS IN FEMALE AND MALE PSEUDOLABRUS SIEBOLDI, A PROTOGYNOUS WRASSE
• Steroidogenic Pathway to Estradiol-17β Synthesis in the Ovarian Follicles of the Protogynous Wrasse, Pseudolabrus sieboldi(Reproductive Biology)
• Environmental Effents on Ethanol Tolerance of Zymomonas mobilis : Ethanol Tolerance of Zymomonas mobilis (I)
• Preparation and Enzymatic Properties of Aspergillus niger Endoinulinase Immobilized onto Various Polysaccharide Supports
• Expression of osteonectin in rat stomach pyloric mucosa after N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) exposure
• An Energy Resolution Formula of a Three Plane Grids Retarding Field Energy Analyzer
• Dendritic Cell Appearance and Differentiation during Early and Late Stages of Rat Stomach Carcinogenesis
• Purification and Characterization of a Novel Serine Proteinase from the Microsomal Fraction of Bovine Pancreas^1
• Characteristics of Raw-Starch-Digesting Glucoamylase from Thermophilic Rhizomucor pusillus
• Presenilin 1 mRNA expression in hippocampi of sporadic Alzheimer's disease patients
• Enzymatic Modification of Glucoamylase of Aspergillus awamori var. kawachi
• An Avicel-affinity Site in an Avicel-digesting Exocellulase from a Trichoderma viride Mutant
• Disruption of ptsG gene and manXYZ operon of ethanol-producing Escherichia coli KO11 : Effects on glucose and xylose utilization and ethanol production(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Ethanol production from non-pretreated napiergrass through a simultaneous saccharification and fermentation process followed by a pentose fermentation with Escherichia coli KO11(BIOCHEMICAL ENGINEERING)
• Disruption of ptsG gene and manXYZ operon of ethanol-producing Escherichia coli KO11 : Effects on glucose and xylose utilization and ethanol production
• Restriction Endonuclease Mapping of pUHl in Bacillus subtilis (natto)
• Relationship between Carbohydrate Moiety and Thermostability of β-Glucosidase from Mucor miehei YH-10
• Characterization of a glycoside hydrolase family-51 α-L-arabinofuranosidase gene from Aureobasidium pullulans ATCC 20524 and its encoded product(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Production and Characterization of Thermostable Xylanase from Talaromyces byssochlamydoides YH-50
• Isolation and characterization of a mutant recombinant Saccharomyces cerevisiae strain with high efficiency xylose utilization(ENVIRONMENTAL BIOTECHNOLOGY)
• Single Nucleotide Polymorphism Analysis of a Trichoderma reesei Hyper-Cellulolytic Mutant Developed in Japan

もっと見る 閉じる

スポンサーリンク