BAC library construction and BAC end sequencing of five Drosophila species : the comparative map with the D. melanogaster genome

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概要

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• We constructed and characterized arrayed bacterial artificial chromosome (BAC) libraries of five Drosophila species (D. melanogaster, D. simulans, D. sechellia, D. auraria, and D. ananassae), which are genetically well characterized in the studies of meiosis, evolution, population genetics, and developmental biology. The BAC libraries comprise 8,000 to 12,500 clones for each species, estimated to cover the most of the genomes. We sequenced both ends of most of these BAC clones with a success rate of 91%. Of these, 53,701 clones consisting of non-repetitive BAC end sequences (BESs) were mapped with reference of the public D. melanogaster genome sequences. The BES mapping estimated that the BAC libraries of D. auraria and D. ananassae covered 47% and 57% of the D. melanogaster genome, respectively, and those of D. melanogaster, D. sechellia, and D. simulans covered 94–97%. The low coverage by BESs of D. auraria and D. ananassae may be due to the high sequence divergence with D. melanogaster. From the comparative BES mapping, 111 possible breakpoints of chromosomal rearrangements were identified in these four species. The breakpoints of the major chromosome rearrangement between D. simulans and D. melanogaster on the third chromosome were determined within 20 kb in 84E and 30 kb in 93E/F. Corresponding breakpoints were also identified in D. sechellia. The BAC clones described here will be an important addition to the Drosophila genomic resources.

• 日本遺伝学会の論文
• 2008-06-25

著者

• Sakaki Y

  Riken Genomic Sciences Center

• Sakaki Y

  Genomic Sciences Center Riken Yokohama Institute

• Hattori Manabu

  Department Of Chemistry College Of Science And Engineering Aoyama Gakuin University

• Toyoda Atsushi

  Riken Genomic Sciences Center

• Toyoda A

  Envysis Co. Ltd. Shikama Jpn

• Toyoda Atsushi

  Department Of Bioengineering Faculty Of Engineering Soka University

• Toyoda Atsushi

  Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture

• Sakaki Yoshiyuki

  Riken Genomic Sciences Center

• MURAKAMI Katsuhiko

  RIKEN Genomic Sciences Center

• HATTORI Masahira

  RIKEN Genomic Sciences Center

• KUROKI Yoko

  RIKEN Genomic Sciences Center

• FUJIYAMA Asao

  RIKEN Genomic Sciences Center

• KOJIMA Toshio

  RIKEN Genomic Sciences Center

• MATSUDA Muneo

  Department of Biology, School of Medicine, Kyorin University

• YAMAMOTO Masa-Toshi

  Drosophila Genetic Resource Center, Kyoto Institute of Technology

• Matsuda Muneo

  Department Of Biology School Of Medicine Kyorin University

• Sakaki Yoshiyuki

  Human Genome Center Institute Of Medical Science University Of Tokyo

• Yamamoto Masa-toshi

  Drosophila Genetic Resource Center Kyoto Institute Of Technology

• Sakaki Y

  Univ. Tokyo Tokyo

• Hattori M

  Department Of Chemistry College Of Science And Engineering Aoyama Gakuin University

• Yamamoto Masa-toshi

  Drosophila Genetic Resource Center Kyoto Inst. Of Technol.

• TOYODA Atsushi

  RIKEN Genome Sciences Center

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