Production of D-[1-^<13>C]Glucose from[^<13>C]Formaldehyde and D-Ribose-5-Phosphate Using an Enzymatic System

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概要

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• D-[1-^<13>C]Glucose is useful for studies on tracing by means of ^<13>C-magnetic resonance imaging(MRI)the fate of a metabolite in the brain, and this technique is expected to become a sophisticated clinical tool for the diagnosis of neurological disorders. For this purpose, a more efficient method of producing inexpensive ^<13>C-labeled D-glucose is necessary, and was investigated. When a mixture of the labeled D-fructose-6-phosphate(F-6-P)and D-glucose-6-phosphate(G-6-P)prepared from D-ribose-5-phosphate and[^<13>C]formaldehyde is dephosphorylated by potato acid phosphatase, a combination of potato acid phosphatase, phosphoglucose isomerase, and BaCl_2 was found to be effective in improving the yield of D-glucose and shortening the reaction time. The maximum yield of labeled D-glucose from the mixture of labeled F-6-P and G-6-P was 84%. Two peaks indicative of labeled D-glucose with ^<13>C incorporated at the C-1 position were confirmed by the NMR spectroscopy.

• 社団法人日本生物工学会の論文
• 1998-05-25

著者

• Toyoda A

  Envysis Co. Ltd. Shikama Jpn

• Toyoda Atsushi

  Department Of Bioengineering Faculty Of Engineering Soka University

• Toyoda Atsushi

  Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture

• MAEDA Hidekatsu

  Department of Bioengineering, Faculty of Engineering, Soka University

• TAKATA KOHHEI

  Department of Bioengineering, Faculty of Engineering, Soka University

• SHIBATA KUNIHIKO

  Frontier Technology Research Institute, Tokyo Gas Co.

• Takata Kohhei

  Department Of Bioengineering Faculty Of Engineering Soka University

• Maeda H

  Soka Univ. Tokyo Jpn

• Shibata K

  Tokyo Gas Co. Yokohama Jpn

• Shibata Kunihiko

  Frontier Technology Research Institute Tokyo Gas Co.

• Maeda Hidekatsu

  Department Of Bioengineering Faculty Of Engineering Soka University

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