Production of L-[3-^<13>C] Serine from [ ^<13>C] formaldehyde and Glycine Using an Enzyme System Combined with Tetrahydrofolate Regeneration

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概要

• 論文の詳細を見る

• ^<13>C-labelled L-serine was efficiently produced from [^<13>C] formaldehyde and glycine by using partially purified L-serine hydroxymethyltransferase from Methylobacterium extorquens JCM 2804,tetrahydrofolate and a tetrahydrofolate regeneration system. The tetrahydrofolate regeneration system allowed efficient L-serine production and direct use of dihydrofolate easily prepared from folic acid. A terahydrofolate concentration of more than 0.020% was necessary ofr efficient production. Eighty seven % of the amount of [^<13>C] formaldehyde consumed, i.e., the difference between the total amount of formaldehyde fed and the amount of formaldehyde remaining in the reaction mixuture, was used for the incorporation into L-[^<13>C] serine. Seventy two mg of added glycine was converted to L-serine when 100 mg of glycine was contained in 11.2 ml of the final reaction mixture.The L-serine was confirmed to be L-[3-^<13>C]-serine by negative fast atom bombarment mass spectrometry (FAB-MS) and ^<13>C-nuclear magnetic resonance spectrometry (NMR) analysis.

• 社団法人日本生物工学会の論文
• 1997-01-25

著者

• Toyoda A

  Envysis Co. Ltd. Shikama Jpn

• Toyoda Atsushi

  Department Of Bioengineering Faculty Of Engineering Soka University

• Toyoda Atsushi

  Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture

• MAEDA Hidekatsu

  Department of Bioengineering, Faculty of Engineering, Soka University

• NIITSU Takashi

  Department of Chemistry, Faculty of Science, the University of Tokyo

• Niitsu T

  Department Of Bioengineering Soka University

• Niitsu Takashi

  Department Of Chemistry Faculty Of Science The University Of Tokyo

• Niitsu Takashi

  Department Of Bioengineering Faculty Of Engineering Soka University

• TAKATA KOHHEI

  Department of Bioengineering, Faculty of Engineering, Soka University

• SHIBATA KUNIHIKO

  Frontier Technology Research Institute, Tokyo Gas Co.

• IWAKURA MASANORI

  National Institute of Bioscience and Human Technology

• Takata Kohhei

  Department Of Bioengineering Faculty Of Engineering Soka University

• Maeda H

  Soka Univ. Tokyo Jpn

• Shibata K

  Tokyo Gas Co. Yokohama Jpn

• Shibata Kunihiko

  Frontier Technology Research Institute Tokyo Gas Co.

• Maeda Hidekatsu

  Department Of Bioengineering Faculty Of Engineering Soka University

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