Cloning, Sequencing, and Characterization of the Intracellular Invertase Gene from Zymomonas mobilis(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
The structural gene for the intracellular invertase E1 of Zymomonas mobilis strain Z6C was cloned in 2.25-kb DNA fragment on pUSH11, and expressed in Escherichia coli HB101. The enzyme produced by the E. coli carrying pUSH11 was purified about 1,122 fold to homogeneity with a yield of 4%. The molecular weight and substrate specificity of the enzyme were identical with those of the intracellular invertase E1 from Z. mobilis. The nucleotides of the cloned DNA were sequenced; they included an open reading frame of 1,536 bp, coding for a protein with a molecular weight of 58,728. The N-terminal amino acid sequence predicted was identical with the sequence of the first 20 N-terminal amino acid residues of the protein obtained by Edman degradation. Comparison of the predicted amino acid sequence of E1 protein with those of the four other known β-D-fructofuranosidases from Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae indicated a stronger homology in the N-terminal portion than in the C-terminal portion.
- 社団法人日本農芸化学会の論文
- 1991-05-23
著者
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YANASE Hideshi
Department of Biotechnology, Faculty of Engineering, Tottori University
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Toyoda A
Envysis Co. Ltd. Shikama Jpn
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Toyoda Atsushi
Department Of Bioengineering Faculty Of Engineering Soka University
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Toyoda Atsushi
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture
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Yanase Hideshi
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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TONOMURA KENZO
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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Ueda Naoki
Department Of Orthopedic Surgery Osaka Medical College
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Tonomura K
Department Of Food Technology Faculty Of Engineering Fukuyama University
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Tonomura Kenzo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Fukushi Hideaki
Department of Agricultural Chemistry, Faculty of Agriculture, University of Osaka Prefecture
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Ueda Naoki
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture
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MAEDA Yukitoshi
Department of Agricultural Chemistry, Faculty of Agriculture, University of Osaka Prefecture
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Fukushi Hideaki
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture
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Maeda Yukitoshi
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Osaka Prefecture
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