Effects of GroESL Coexpression on the Folding of Nicotinoprotein Formaldehyde Dismutase from Pseudomonas putida F61(Microbiology & Fermentation Technology)

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概要

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• The overexpression of fdm, which encodes the formaldehyde dismutase from Pseudomonas putida F61, resulted in the formation of inclusion bodies made up of aggregated enzyme, leaving little activity in the soluble fraction of the transformant cells. On the other hand, coexpression of groESL along with fdm facilitated in vivo solubilization of the enzyme protein in its active form. When coexpressed with groESL, formaldehyde dismutase purified from E. coli had the same crystalline form (i. e., a regular octahedron) as the native enzyme, and like the native enzyme, it bound 1 mol of NAD (H) and 2 mol of zinc in each subunit.

• 社団法人日本農芸化学会の論文
• 2002-01-23

著者

• Yanase Hideshi

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Okamoto Kenji

  Department Of Applied Physics Osaka University

• Kato Nobuo

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University:(present Address)k

• Kawata Yasushi

  Department Of Biotechnology Faculty Of Engineering Tottori University

• MORIYA Keishi

  Department of Biotechnology, Faculty of Engineering, Tottori University

• MUKAI Norihiko

  Department of Biotechnology, Faculty of Engineering, Tottori University

• Moriya Keishi

  Department Of Biotechnology Faculty Of Engineering Tottori University

• Mukai Norihiko

  Department Of Biotechnology Faculty Of Engineering Tottori University

• Kato Nobuo

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• KAWATA Yasushi

  Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University

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