Development of Biorational Pest Control Formulation against Longicorn Beetles Using a Fungus, Beauveria brongniartii (Sacc.) Petch

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概要

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• Some basic experiments were performed in order to develop a biocidal formulation which is lethal to certain species of longicorn beetle. The entomopathogenic filamentous fungus, Beauveria brongniartii could be grown aerobically in the presence of 4% corn steep liquor and 2% glucose at 25℃ with a minimum doubling time of 7 h. Depletion of glucose and the onset of the stationary phase occurred within 48 h. Application of a diluted aliquot of this culture to a nonwoven pulp fabric sheet resulted in the growth of B. broungniartii on the surface of the fabric. The conditions for the growth of cultures on fabric sheets were optimized and large-scale cultivation of the cultures on fabric was achieved in a specially designed chamber. The cultures covered with conidia could be stored sealed in polyethylene bags for more than 400 d at 5℃ without loss of infectivity, while storage at room temperature resulted in a gradual decrease in the number of viable cells. Infectivity was lost when the population of cells decreased below 10^7 cells/cm^2. However, when water was added to dried cultures which had been stored at 25℃ for an extended period of time, the number of viable cells recovered and the infectivity of the cultures could be restored. Under field conditions, periodic rainfall which moistens the sheet cultures may help to sustain the infectivity of this agent. When the effects of this biocide were probed in field tests using tangerine crops, it was observed that the use of this agent prevented the proliferation of beetles, in addition to killing adult insects.

• 社団法人日本生物工学会の論文
• 1997-09-25

著者

• Nagai J

  Tottori Univ. Tottori Jpn

• Higuchi Tosho

  Medical Product Division Product Development Center Nitto Denko:department Of Biotechnology Tottori

• Nagai Jun

  Department of Biotechnology, Tottori University

• Kawata Yasushi

  Department Of Biotechnology Tottori University

• Kawata Yasushi

  Department Of Biotechnology Faculty Of Engineering Tottori University

• SENDA SHUJI

  Medical Research Center, Nitto Denko Corporation

• MIZOBATA Tomohiro

  Department of Chemistry, Faculty of Science, Kyoto University

• HIGUCHI TOSHIO

  Medical Product Division, Product Development Center, Nitto Denko

• SAIKA TAKESHI

  Medical Product Division, Product Development Center, Nitto Denko

• Senda Shuji

  Medical Product Division Product Development Center Nitto Denko

• Mizobata T

  Department Of Biotechnology Tottori University

• Mizobata Tomohiro

  Department Of Chemistry Faculty Of Science Kyoto University

• Nagai Jun

  Department Of Biotechnology Faculty Of Engineering Tottori University

• Mizobata Tomohiro

  Department of Biotechnology, Tottori University

• KAWATA Yasushi

  Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University

• MIZOBATA Tomohiro

  Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University

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