Chalcone and Stilbene Synthases Expressed in Eucaryotes Exhibit Reduced Cross-Reactivity in Vitro

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概要

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• Chalcone synthase (CHS) and stilbene synthase (STS) catalyze different cyclization reactions of the common tetraketide to give different products, naringenin chalcone and resveratrol, respectively. We have previously observed in vitro cross-reaction of CHS and STS overexpressed in Escherichia coli, resveratrol production by CHS and chalcone production by STS. When expressed in eucaryotic cells, or in E. coli as thioredoxin-fusion proteins, CHS and STS exhibited reduced cross-reaction. STS refolded from inclusion bodies also showed reduced cross-reaction. While addition of bovine serum albumin and pH in the reaction were without noticeable effect, addition of glycerol decreased the cross-reaction of CHS likely due to its stabilizing effect on enzyme conformation. These results were interpreted to provide supporting evidence to our earlier proposition (Yamaguchi T. et al., FEBS Lett., 460,457-461 (1999)) that the in vitro cross-reaction of CHS and STS is due to intrinsic capability of these enzymes to catalyze different types of cyclization, which, in turn, is endowed by conformational flexibility of their active sites.

• 公益社団法人日本薬学会の論文
• 2000-07-01

著者

• Sakai Yasuyoshi

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yurimoto Hiroya

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yurimoto Hiroya

  Department Of Agricultural Chemistry And Department Of Wood Science And Technology Faculty Of Agricu

• 三川 潮

  富山医薬大薬

• 三川 潮

  Faculty Of Pharmaceutical Sciences University Of Tokyo

• Sakai Yasuyoshi

  Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.

• 加藤 暢夫

  鳥取大・工

• Ebizuka Yutaka

  Graduate School of Pharmaceutical Sciences, The University of Tokyo

• KATO NOBUO

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• SUH Dae-Yeon

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• IWANAMI Naoko

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• SANKAWA Ushio

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• Sankawa U

  International Research Center For Traditional Medicine

• Sankawa Ushio

  (present Address)faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• Sankawa Ushio

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• Sankawa Ushio

  Faculty Of Pharmaceautical Sciences University Of Tokyo

• 加藤 暢夫

  鳥取大学、工

• Iwanami N

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• Iwanami Naoko

  Faculty Of Pharmaceutical Sciences The University Of Tokyo

• Yamazaki Yohko

  微生物化学研究会微生物化学研究センター沼津創薬医科学研究所

• KAGAMI Junichi

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• FUKUMA Kazuki

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• YAMAZAKI Yasuyo

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• SHIBUYA Masaski

  Graduate School of Pharmaceutical Sciences, The University of Tokyo

• Kato Nobuo

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University:(present Address)k

• Suh Dae-yeon

  Department Of Chemistry And Biochemistry University Of Regina

• Fukuma Kazuki

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• Ebizuka Yutaka

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Kagami Junichi

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• Shibuya Masaski

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Yamazaki Yasuyo

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• 山嵜 行由

  School Of Pharmaceutical Sciences University Of Shizuoka

• Kato Nobuo

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Suh Dae-yeon

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

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