The Peroxisomal Catalase Gene in the Methylotrophic Yeast Pichia methanolica

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概要

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• In this paper, we describe the CTA1 gene, which encodes a peroxisomal catalase in the methylotrophic yeast Pichia methanolica. The P. methanolica CTA1 gene (PmCTA1) comprises a 1,530-bp open reading frame corresponding to a protein of 510 amino acid residues, and its deduced amino acid sequence shows high similarity to those of Cta1ps from other methylotrophic yeasts (about 79%). Expression of PmCTA1 in a peroxisomal catalase-depleted (Cbcta1Δ) Candida boidinii strain restored the methylotrophic growth of the host strain, while the expression of PmCTA1-ΔSRL, which lacks peroxisome targeting signal type 1, did not. In P. methanolica, expression of PmCTA1 was induced when cells were grown on peroxisome-inducing carbon sources, viz., methanol, oleate, and D-alanine. Taken together, these results indicate that PmCTA1 encodes a functional peroxisomal catalase in P. methanolica.

• 社団法人 日本農芸化学会の論文

著者

• Sakai Yasuyoshi

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yurimoto Hiroya

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Ito Takashi

  Department Of Physics University Of Tokyo

• TOMIZUKA NOBORU

  Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture

• Nakagawa Tomoyuki

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yoshida Kyoko

  Department Of Biology Faculty Of Science Hirosaki University

• NAKAGAWA Tomoyuki

  Faculty of Applied Biological Science, Gifu University

• TAKEUCHI Akihito

  Faculty of Applied Biological Science, Gifu University

• FUJIMURA Shuki

  Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture

• MATSUFUJI Yoshimi

  Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture

• HAYAKAWA Takashi

  Faculty of Applied Biological Science, Gifu University

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