Activation of the Oxidative Stress Regulator PpYap1 through Conserved Cysteine Residues during Methanol Metabolism in the Yeast Pichia pastoris

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概要

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• The methylotrophic yeast Pichia pastoris can grow on methanol as sole source of carbon and energy. The first reaction in yeast methanol metabolism, catalyzed by an abundant peroxisomal enzyme, alcohol oxidase, generates high levels of H2O2, but the oxidative stress response during methanol metabolism has not been elucidated. In this study, we isolated the Yap1 homolog of P. pastoris (PpYap1) and analyzed the properties of a PpYAP1-disruption strain. The PpYap1 transcription factor is activated after exposure to various reactive agents, and therefore functions as a regulator of the redox system in P. pastoris. We have also identified PpGPX1, the unique glutathione peroxidase-encoding gene in P. pastoris whose expression is induced by PpYap1. PpGpx1, but not the ScTsa1 or SpTpx1 homolog PpTsa1, functions as a H2O2 sensor and activates PpYap1. This study is the first demonstration of a yeast Yap1 family protein activated during conventional metabolism.

• 社団法人 日本農芸化学会の論文
• 2009-06-23

著者

• Sakai Yasuyoshi

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yurimoto Hiroya

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Sakai Yasuyoshi

  Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.

• Yano Taisuke

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• Yano Taisuke

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

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