Hyper-Activation of the Target of Rapamycin (Tor) Kinase 1 Decreases Intracellular Glutathione Content in Saccharomyces cerevisiae as Revealed by LC-MS/MS Analysis

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概要

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• The targets of rapamycin (Tor) kinases play central roles in the integrated regulation of cellular activities. Although the molecular mechanisms of Tor-mediated signaling pathways have been studied extensively in yeast, the relationship between kinase activity and the redox maintenance system remains obscure. In this study, we established a quantitative extraction and determination method for glutathione-related compounds in Saccharomyces cerevisiae utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS). We found decreases in the levels of glutathione and its precursors resulting from the introduction of a Tor1 hyper-active mutation. In line with this finding, the mutant was more sensitive to several heavy metal ions, indicating a physiological defect arising from a failure to regulate the kinase activity.

著者

• Sakai Yasuyoshi

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Maeda Tatsuya

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Ichiki Yayoi

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• OKU Masahide

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• SHIRAISHI Akiko

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

• TAKAHARA Terunao

  Institute of Molecular and Cellular Bioscences, The University of Tokyo

• MAEDA Tatsuya

  Institute of Molecular and Cellular Bioscences, The University of Tokyo

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