Activation of the Oxidative Stress Regulator PpYap1 through Conserved Cysteine Residues during Methanol Metabolism in the Yeast Pichia pastoris

スポンサーリンク

概要

• 論文の詳細を見る

• The methylotrophic yeast Pichia pastoris can grow on methanol as sole source of carbon and energy. The first reaction in yeast methanol metabolism, catalyzed by an abundant peroxisomal enzyme, alcohol oxidase, generates high levels of H2O2, but the oxidative stress response during methanol metabolism has not been elucidated. In this study, we isolated the Yap1 homolog of P. pastoris (PpYap1) and analyzed the properties of a PpYAP1-disruption strain. The PpYap1 transcription factor is activated after exposure to various reactive agents, and therefore functions as a regulator of the redox system in P. pastoris. We have also identified PpGPX1, the unique glutathione peroxidase-encoding gene in P. pastoris whose expression is induced by PpYap1. PpGpx1, but not the ScTsa1 or SpTpx1 homolog PpTsa1, functions as a H2O2 sensor and activates PpYap1. This study is the first demonstration of a yeast Yap1 family protein activated during conventional metabolism.

• 社団法人 日本農芸化学会の論文

著者

• Sakai Yasuyoshi

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Yurimoto Hiroya

  Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University

• Sakai Yasuyoshi

  Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.

• Yano Taisuke

  Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

関連論文

• 2-I-18 メタノール資化性細菌におけるPQQ生産性と植物生育促進効果(一般研究発表,日本ビタミン学会第61回大会研究発表要旨)
• Assimilation of Formaldehyde in Transgenic Plants Due to the Introduction of the Bacterial Ribulose Monophosphate Pathway Genes
• 2-II-30メタン資化性細菌の生育を促進する根粒菌由来のバイオファクター(一般演題,日本ビタミン学会第62回大会発表要旨)
• Molecular Characterization of Two Genes with High Similarity to the Dihydroxyacetone Synthase Gene in the Methylotrophic Yeast Pichia methanolica
• P-92 水生植物に生息するメタン資化性細菌叢解析(ポスター発表)
• 自然界での生存戦略からみたオートファジーと糸状菌の植物病原性 : To be, or not to be : that is the question……
• 2Da09 メタノール資化性酵母におけるホルムアルデヒドデヒドロゲナーゼ高発現株の解析(遺伝子工学,核酸工学,一般講演)
• 1Cp19 Pichia methanolicaの2種のジヒドロキシアセトンシンターゼの機能解析(代謝工学・メタボローム,一般講演)
• C_1微生物と植物の相互作用と炭素循環 : 代謝生化学・分子細胞生物学からの理解
• 3P-1130 メチロトローフ酵母Pichia methanolicaのジヒドロキシアセトンシンターゼと相同性が高い2つの遺伝子の機能解析(3b代謝工学,一般講演,代謝生理学・発酵生産,伝統の技と先端科学技術の融合)
• Molecular analysis of α-thalassemia in Nepal : correlation with malaria endemicity
• The Physiological Role of the Ribulose Monophosphate Pathway in Bacteria and Archaea
• 3P-1131 メチロトローフ酵母Pichia methanolica Cta1pのペルオキシソーム酵素としての機能と発現誘導(3b代謝工学,一般講演,代謝生理学・発酵生産,伝統の技と先端科学技術の融合)
• Two Acyl-CoA Dehydrogenases of Acinetobacter sp. Strain M-1 That Uses Very Long-Chain n-Alkanes
• Purification and Properties of Cephalosporin-C Deacetylase from the Yeast, Rhodotorula glutinis 38B1, Useful for Bioconversion of 7-Aminocephalosporanic Acid Derivatives
• Ester Synthesis by NAD^+ -dependent Dehydrogenation of Hemiacetal : Production of Methyl Formate by Cells of Methylotrophic Yeasts
• A Non-Conventional Dissimilation Pathway for Long Chain n-Alkanes in Acinetobacter sp. M-1 That Starts with a Dioxygenase Reaction
• Production of Fructosyl Lysine Oxidase from Fusarium oxysporum S-1F4 on Autoclave-browned Medium
• Purification and Properties of FructosylLysine Oxidase from Fusarium oxysporum S-1F4
• Use of Long-chain n-Alkanes (C_13-C_44) by an Isolate, Acinetobacter sp. M-1
• 2Ba09 メタン資化性菌のメタン酸化を促進するコンソーシアムの解析(環境浄化・修復・保全技術,一般講演)
• 2H09-1 メタン資化性菌とメタノール資化性菌からなるコンソーシアのメタン消費(環境浄化・修復・保全技術,一般講演)
• 光合成経路と相性のよいホルムアルデヒド固定反応
• 生物と工学のギャップを埋める"生命の神秘"(随縁随意)
• Association analysis of polymorphisms in the upstream region of the human dopamine D4 receptor gene (DRD4) with schizophrenia and personality traits
• アーキアにおけるホルムアルデヒド固定酵素群の生理機能
• 3E09-2 Candida boidiniiホルムアルデヒドデヒドロゲナーゼ遺伝子プロモーターの活性化制御(遺伝子工学・核酸工学,一般講演)
• 3E09-3 酵母メタノール誘導性プロモーターの転写活性化因子の探索(遺伝子工学・核酸工学,一般講演)
• 3E10-1 酵母メタノール代謝におけるストレス応答性転写因子の役割(遺伝子工学・核酸工学,一般講演)
• 未来型資源の利用を目指すC1微生物 (特集/新しい化学技術の挑戦と未来)
• 酵母ミクロペキソファジーの細胞内ダイナミクス
• Gene-Tagging Mutagenesis in the Methylotrophic Yeast Candida boidinii(METHODS)
• Molecular Basis of Methanol-Inducible Gene Expression and Its Application in the Methylotrophic Yeast Candida boidinii
• Gene Structure and Regulation of Alkane Monooxygenases in Propane-Utilizing Mycobacterium sp. TY-6 and Pseudonocardia sp. TY-7(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• The Pro-peptide of Streptomyces mobaraensis Transglutaminase Functions in cis and in trans to Mediate Efficient Secretion of Active Enzyme from Methylotrophic Yeasts
• Characterization and High-level Production of D-Amino Acid Oxidase in Candida boidinii
• Analysis of Alcohol Oxidase Isozymes in Gene-Disrupted Strains of Methylotrophic Yeast Pichia methanolica
• Chalcone and Stilbene Synthases Expressed in Eucaryotes Exhibit Reduced Cross-Reactivity in Vitro
• A New Carboxylesterase from Brevibacterium linens IFO 12171 Responsible for the Conversion of 1,4-Butanediol Diacrylate to 4-Hydroxybutyl Acrylate : Purification, Characterization, Gene Cloning, and Gene Expression in Escherichia coli
• Subcellular Localization of Fructosyl Amino Acid Oxidases in Peroxisomes of Aspergillus terreus and Penicillium janthinellum
• A Novel Arylesterase Active toward 7-Aminocephalosporanic Acid from Agrobacterium radiobdcter IFO 12607 : Nucleotide Sequence, Gene Expression in Escherichia coli, and Site-Directed Mutagenesis
• A Novel Arylesterase Active toward 7-Aminocephalosporanic Acid from Agrobacterium radiobacter IFO 12607 : Purification and Characterization
• Isolation and Characterization of Mutants of the Methylotrophic Yeast, Candida boidinii S2 That Are Impaired in Growth on Peroxisome-Inducing Carbon Sources
• Activation of the Oxidative Stress Regulator PpYap1 through Conserved Cysteine Residues during Methanol Metabolism in the Yeast Pichia pastoris
• Position-independent human β-globin gene expression mediated by a recombinant adeno-associated virus vector carrying the chicken β-globin insulator
• A Front-end Desaturase from Chlamydomonas reinhardtii Produces Pinolenic and Coniferonic Acids by ω13 Desaturation in Methylotrophic Yeast and Tobacco
• The Peroxisomal Catalase Gene in the Methylotrophic Yeast Pichia methanolica
• A Novel Hemiacetal Dehydrogenase Activity Involved in Ethyl Acetate Synthesis in Candida utilis
• Hemiacetal Dehydrogenation Activity of Alcohol Dehydrogenases in Saccharomyces cerevisiae
• The Peroxisomal Catalase Gene in the Methylotrophic Yeast Pichia methanolica
• Molecular Characterization of Two Genes with High Similarity to the Dihydroxyacetone Synthase Gene in the Methylotrophic Yeast Pichia methanolica
• Assimilation of Formaldehyde in Transgenic Plants Due to the Introduction of the Bacterial Ribulose Monophosphate Pathway Genes
• Characterization of a New (R)-Hydroxynitrile Lyase from the Japanese Apricot Prunus mume and cDNA Cloning and Secretory Expression of One of the Isozymes in Pichia pastoris
• Activation of the Oxidative Stress Regulator PpYap1 through Conserved Cysteine Residues during Methanol Metabolism in the Yeast Pichia pastoris
• Intracellular ATP Correlates with Mode of Pexophagy in Pichia pastoris
• Construction of Protease-deficient Candida boidinii Strains Useful for Recombinant Protein Production : Cloning and Disruption of Proteinase A Gene (PEP4) and Proteinase B Gene (PRB1)(Microbiology & Fermentation Technology)
• Anaerobic Degradation of Aromatic Compounds by Magnetospirillum Strains: Isolation and Degradation Genes
• Molecular Basis of Methanol-Inducible Gene Expression and Its Application in the Methylotrophic Yeast Candida boidinii
• Msn5p Is Involved in Formaldehyde Resistance but Not in Oxidative Stress Response in the Methylotrophic Yeast Candida boidinii
• Distribution of Pink-Pigmented Facultative Methylotrophs on Leaves of Vegetables
• Distribution of Pink-Pigmented Facultative Methylotrophs on Leaves of Vegetables
• Distribution of Methanotrophs in the Phyllosphere
• Hyper-Activation of the Target of Rapamycin (Tor) Kinase 1 Decreases Intracellular Glutathione Content in Saccharomyces cerevisiae as Revealed by LC-MS/MS Analysis
• Dominant Colonization and Inheritance of Methylobacterium sp. Strain OR01 on Perilla Plants
• Dominant Colonization and Inheritance of Methylobacterium sp. Strain OR01 on Perilla Plants

もっと見る 閉じる

スポンサーリンク