Analysis of Alcohol Oxidase Isozymes in Gene-Disrupted Strains of Methylotrophic Yeast Pichia methanolica
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概要
- 論文の詳細を見る
A cell-free extract of methanol-grown Pichia methanolica cells was found to contain nine alcohol oxidase (AOD) isozymes by active staining of a native polyacrylamide electrophoresis gel. Our previous study revealed that AOD in P.methanolica was encoded by two genes, MOD1 and MOD2, and the results of an experiment involving Candida boidinii as an expression host suggested that the AOD isozymes observed in P.methanolica were due to random association of Mod1p and Mod2p into an active octamer [Nakagawa et al., Yeast, 15, 1223-1230 (1999)]. This study was conducted using P.methanolica MOD1- and/or MOD2-gene disrupted strains to confirm a previous hypothesis. While the cell-free extract of the wild-type strain gave nine ladder bands, the mod1Δ and mod2Δ strains gave a single active AOD band corresponding to the mobilities of Mod2p and Mod1p on a native electrophoresis gel, respectively. The cell-free extract of glycerol-grown wild-type cells gave a single band corresponding to Mod1p, showing that only MOD1 is expressed in glycerol-grown cells. While the expression of both MOD1 and MOD2 was induced by methanol, this finding and our previous observations indicated that the expression of MOD1 and MOD2 was controlled by a distinct regulatory mechanism in P.methanolica.
- 社団法人日本生物工学会の論文
- 2001-02-25
著者
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Sakai Yasuyoshi
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Sakai Yasuyoshi
京都大学 農学研究科応用生命科学科
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Yurimoto Hiroya
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Yurimoto Hiroya
Department Of Agricultural Chemistry And Department Of Wood Science And Technology Faculty Of Agricu
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Sakai Yasuyoshi
Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Univ.
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加藤 暢夫
鳥取大・工
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NAKAGAWA Tomoyuki
Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University
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Kato N
Institute Of Scientific And Industrial Research Osaka University
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Kato Nobuo
Departments Of Biotechnology Faculty Of Engineering Tottori University
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KATO NOBUO
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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加藤 暢夫
鳥取大学、工
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MUKAIYAMA HIROYUKI
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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MIZUMURA TASUKU
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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MIYAJI TATSURO
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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TOMIZUKA NOBORU
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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Kato Nobuo
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University:(present Address)k
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Tomizuka Noboru
National Institute of Bioscience and Human-Technology (NIBH)
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Nakagawa Tomoyuki
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Mukaiyama Hiroyuki
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Miyaji Tatsuro
Department Of Bioproduction Science School Of Horticulture Chiba University:(present Address) Depart
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Nakagawa Tomoyuki
Department Of Chemistry And Biochemistry Graduate School Of Engineering Kyushu University
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Kato Nobuo
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Mizumura Tasuku
Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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